Journal article
Single-molecule epitranscriptomic analysis of full-length HIV-1 RNAs reveals functional roles of site-specific m 6 As
Nature microbiology, Vol.9(5), pp.1340-1355
04/11/2024
DOI: 10.1038/s41564-024-01638-5
PMCID: PMC11087264
PMID: 38605174
Abstract
Although the significance of chemical modifications on RNA is acknowledged, the evolutionary benefits and specific roles in human immunodeficiency virus (HIV-1) replication remain elusive. Most studies have provided only population-averaged values of modifications for fragmented RNAs at low resolution and have relied on indirect analyses of phenotypic effects by perturbing host effectors. Here we analysed chemical modifications on HIV-1 RNAs at the full-length, single RNA level and nucleotide resolution using direct RNA sequencing methods. Our data reveal an unexpectedly simple HIV-1 modification landscape, highlighting three predominant N
-methyladenosine (m
A) modifications near the 3' end. More densely installed in spliced viral messenger RNAs than in genomic RNAs, these m
As play a crucial role in maintaining normal levels of HIV-1 RNA splicing and translation. HIV-1 generates diverse RNA subspecies with distinct m
A ensembles, and maintaining multiple of these m
As on its RNAs provides additional stability and resilience to HIV-1 replication, suggesting an unexplored viral RNA-level evolutionary strategy.
Details
- Title: Subtitle
- Single-molecule epitranscriptomic analysis of full-length HIV-1 RNAs reveals functional roles of site-specific m 6 As
- Creators
- Alice Baek - The Ohio State UniversityGa-Eun Lee - The Ohio State UniversitySarah Golconda - The Ohio State UniversityAsif Rayhan - University of CincinnatiAnastasios A Manganaris - The Ohio State UniversityShuliang Chen - The Ohio State UniversityNagaraja Tirumuru - The Ohio State UniversityHannah Yu - The Ohio State UniversityShihyoung Kim - The Ohio State UniversityChristopher Kimmel - The Ohio State UniversityOlivier Zablocki - The Ohio State UniversityMatthew B Sullivan - The Ohio State UniversityBalasubrahmanyam Addepalli - University of CincinnatiLi Wu - Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, USASanggu Kim - The Ohio State University
- Resource Type
- Journal article
- Publication Details
- Nature microbiology, Vol.9(5), pp.1340-1355
- DOI
- 10.1038/s41564-024-01638-5
- PMID
- 38605174
- PMCID
- PMC11087264
- NLM abbreviation
- Nat Microbiol
- eISSN
- 2058-5276
- Grant note
- DE-SC0023307 / U.S. Department of Energy (DOE) R01HG010318 / U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (NHGRI) HT9425-23-1-0582 / U.S. Department of Defense (United States Department of Defense) R21AI170070 / U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID) R61AI169659 / U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID) 248445 / U.S. Department of Energy (DOE) HG010108 / U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (NHGRI)
- Language
- English
- Electronic publication date
- 04/11/2024
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984584906802771
Metrics
14 Record Views