Journal article
Single nucleotide polymorphisms of promoter of human leukocyte antigen-DQB1 alleles in Chinese Han patients with Vogt-Koyanagi-Harada syndrome
Chung-hua yen k'o tsa chih, Vol.44(10), pp.870-875
10/01/2008
DOI: 10.3321/j.issn:0412-4081.2008.10.003
PMID: 19176112
Abstract
OBJECTIVETo investigate the single nucleotide polymorphism of the promoter of HLA-DQB1(QBP) in Chinese Han patients with Vogt-Koyanagi-Harada syndrome.METHODSCase-control design was applied. Eighty-eight Chinese Han patients with Vogt-Koyanagi-Harada syndrome and 88 non-Vogt-Koyanagi-Harada syndrome controls were admitted. DNA was extracted from the peripheral white blood cells of the subjects by the phenol-chloroform method. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and clone-sequencing were applied to determine the sequences of the promoter of HLA-DQB1. Chromans and Bioedit software were used to analyze the sequences of the promoter of HLA-DQB1. Chi-square test and Fisher exact test were applied to compare the frequencies of bands of QBPs and SNPs for the two groups.RESULTSSixteen band patterns of HLA-QBP were shown by polyacrylamide gel electrophoresis (PAGE). The band frequencies of QBPb (corresponding gene sequence was QBP2.1 + 77C > A, chi2 = 26.01, Pc < 0.001) and QBP
Details
- Title: Subtitle
- Single nucleotide polymorphisms of promoter of human leukocyte antigen-DQB1 alleles in Chinese Han patients with Vogt-Koyanagi-Harada syndrome
- Creators
- Han-yi MinNi-fang NiuYing LiuMei-fen ZhangXi-lin ZhuJia-liang Zhao
- Resource Type
- Journal article
- Publication Details
- Chung-hua yen k'o tsa chih, Vol.44(10), pp.870-875
- DOI
- 10.3321/j.issn:0412-4081.2008.10.003
- PMID
- 19176112
- NLM abbreviation
- Zhonghua Yan Ke Za Zhi
- ISSN
- 0412-4081
- Number of pages
- 6
- Language
- Chinese
- Date published
- 10/01/2008
- Academic Unit
- Stead Family Department of Pediatrics; Medical Genetics and Genomics
- Record Identifier
- 9984701547402771
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