Journal article
Single treatment with cisplatin or UFT, but not their combination treatment enhances the metastatic capacity of mouse fibrosarcoma cells
Anti-cancer drugs, Vol.10(2), pp.235-244
02/1999
DOI: 10.1097/00001813-199902000-00013
PMID: 10211555
Abstract
To elucidate the roles of chemotherapeutic agents in tumor progression, we examined whether cis-diamminedichloro-platinum (II) (cisplatin) and UFT would promote malignant progression of a weakly tumorigenic and poorly metastatic fibrosarcoma cell line QR-32SK in vivo. C57BL/6 mice, transplanted with QR32SK, were treated with either cisplatin or UFT alone and in combination. After treatment with or without cisplatin and/or UFT, we established in vitro culture cell lines from the tumors arising in the mice on day 21 and then i.v. injected the established cell lines into syngeneic mice. As a result, the cell lines established from cisplatintreated mice and UFT-treated mice had significantly higher metastatic capacity in lung compared to the ones from control untreated mice (64 and 65%, respectively, versus 26.7%). The cell lines established from the mice with the combination therapy showed lower lung metastasis (11%) than the ones from control untreated mice. Furthermore, we found the incidences of these experimental metastases were closely related with in vitro chemotactic activities and the production of MMP-9 of the cultured cell lines. These results indicate that cisplatin and UFT as a single agent promote the chemotactic activities and the production of MMP-9 in non-metastatic fibrosarcoma cells, resulting in the conversion to highly metastatic ones, and that cisplatin and UFT in combination failed to promote the chemotactic activities and the conversion. These results suggest that the combination therapy with cisplatin and UFT is useful in preventing the emergence of more malignant tumor cells after chemotherapy.
Details
- Title: Subtitle
- Single treatment with cisplatin or UFT, but not their combination treatment enhances the metastatic capacity of mouse fibrosarcoma cells
- Creators
- Sungki Choi - Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan. Department of Oral and Maxillo-facial Surgery II, Hokkaido University School of Dentistry, Kita-13, Nishi-7, Kita-ku, Sapporo 060-8586, Japan. Department of Oncological Pathology, Cancer Center Nara Medical University, Kashihara 634-8521, JapanFutoshi OkadaMasanobu KobayashiHasem HabelhahDai NakaeYoichi KonishiYasunori TotsukaMasuo Hosokawa
- Resource Type
- Journal article
- Publication Details
- Anti-cancer drugs, Vol.10(2), pp.235-244
- Publisher
- Lippincott Williams & Wilkins, Inc
- DOI
- 10.1097/00001813-199902000-00013
- PMID
- 10211555
- ISSN
- 0959-4973
- eISSN
- 1473-5741
- Language
- English
- Date published
- 02/1999
- Academic Unit
- Pathology
- Record Identifier
- 9984047881302771
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