Site-specific accumulation of recently activated CD4+Foxp3+ regulatory T cells following adoptive transfer

Scott M Lieberman; Jiyeon S Kim; Evann Corbo-Rodgers; Taku Kambayashi; Jonathan S Maltzman; Edward M Behrens; Laurence A Turka

doi:10.1002/eji.201142286

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Site-specific accumulation of recently activated CD4+Foxp3+ regulatory T cells following adoptive transfer

Journal article

Open access

Peer reviewed

Site-specific accumulation of recently activated CD4+Foxp3+ regulatory T cells following adoptive transfer

Scott M Lieberman, Jiyeon S Kim, Evann Corbo-Rodgers, Taku Kambayashi, Jonathan S Maltzman, Edward M Behrens and Laurence A Turka

European journal of immunology, Vol.42(6), pp.1429-1435

06/2012

DOI: 10.1002/eji.201142286

PMCID: PMC3664195

PMID: 22678899

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Abstract

CD4 + Foxp3 + regulatory T cells (Tregs) are required for maintenance of self-tolerance, as demonstrated by profound autoimmunity in mice and humans with inactivating Foxp3 mutations. Recent studies demonstrate that Tregs are anatomically compartmentalized within secondary lymphoid organs based on their TCR repertoire and specific organ-protective function; however, whether this reflects differential homing or in situ selection is not known. Here, using Foxp3-GFP reporter mice, we have examined the ability of polyclonal Tregs from cervical LN to return to their site-of-origin following adoptive transfer to non-lymphopenic congenic recipients. We find that bulk cervical LN Tregs do not home directly to cervical LN but, rather, accumulate site-specifically over time following transfer. Site-specific enrichment is both more rapid and more pronounced among a population of recently activated (CD69 + ) Tregs. These data suggest that compartmentalization of Tregs within secondary lymphoid organs may be governed by antigen recognition and implicate CD69 as a potential marker of recently activated Tregs recognizing local-expressed antigens.

cell trafficking

T cell receptor

lymph nodes

Regulatory T cells

cellular immunology

Details

Title: Subtitle: Site-specific accumulation of recently activated CD4+Foxp3+ regulatory T cells following adoptive transfer
Creators: Scott M Lieberman - Division of Rheumatology, The Children’s Hospital of Philadelphia, Philadelphia, PA
Jiyeon S Kim - Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA
Evann Corbo-Rodgers - Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA
Taku Kambayashi - Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA
Jonathan S Maltzman - Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA
Edward M Behrens - Division of Rheumatology, The Children’s Hospital of Philadelphia, Philadelphia, PA
Laurence A Turka - The Transplant Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA
Resource Type: Journal article
Publication Details: European journal of immunology, Vol.42(6), pp.1429-1435
DOI: 10.1002/eji.201142286
PMID: 22678899
PMCID: PMC3664195
NLM abbreviation: Eur J Immunol
ISSN: 0014-2980
eISSN: 1521-4141
Language: English
Date published: 06/2012
Academic Unit: Stead Family Department of Pediatrics; Rheumatology, Allergy, and Immunology
Record Identifier: 9984093372502771

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