Journal article
Site-specific characteristics of bone and progenitor cells in control and ovariectomized rats
Bone (New York, N.Y.), Vol.163, pp.116501-116501
10/01/2022
DOI: 10.1016/j.bone.2022.116501
PMID: 35872108
Abstract
One-third of postmenopausal women experience at least one osteoporotic bone fracture in their lifetime that occurs spontaneously or from low-impact events. However, osteoporosis-associated jaw bone fractures are extremely rare. It was also observed that jaw bone marrow stem cells (BMSCs) have a higher capacity to form mineralized tissues than limb BMSCs. At present, the underlying causes and mechanisms of variations between jaw bone and limb bone during postmenopause are largely unknown. Thus, the objective of the current study was to examine the site-specific effects of estrogen deficiency using comprehensive analysis of bone quantity and quality, and its association with characterization of cellular components of bone. Nine rats (female, 6 months old) for each bilateral sham and ovariectomy (OVX) surgery were obtained and maintained for 2 months after surgery. A hemi-mandible and a femur from each rat were characterized for parameters of volume, mineral density, cortical and trabecular morphology, and static and dynamic mechanical analysis. Another set of 5 rats (female, 9 months old) was obtained for assays of BMSCs. Following cytometry to identify BMSCs, bioassays for proliferation, and osteogenic, adipogenic, chondrogenic differentiation, and cell mitochondrial stress tests were performed. In addition, mRNA expression of BMSCs was analyzed. OVX decreased bone quantity and quality (mineral content, morphology, and energy dissipation) of femur while those of mandible were not influenced. Cellular assays demonstrated that mandible BMSCs showed greater differentiation than femur BMSCs. Gene ontology pathway analysis indicated that the mandibular BMSCs showed most significant differential expression of genes in the regulatory pathways of osteoblast differentiation, SMAD signaling, cartilage development, and glucose transmembrane transporter activity. These findings suggested that active mandibular BMSCs maintain bone formation and mineralization by balancing the rapid bone resorption caused by estrogen deficiency. These characteristics likely help reduce the risk of osteoporotic fracture in postmenopausal jawbone.
Details
- Title: Subtitle
- Site-specific characteristics of bone and progenitor cells in control and ovariectomized rats
- Creators
- Jie Liu - The Ohio State UniversityKeiichiro Watanabe - The Ohio State UniversityShareef M. Dabdoub - Univ Iowa, Coll Dent & Dent Clin, Dept Periodont, Div Biostat & Computat Biol, Iowa City, IA 52242 USABeth S. Lee - The Ohio State UniversityDo-Gyoon Kim - The Ohio State University
- Resource Type
- Journal article
- Publication Details
- Bone (New York, N.Y.), Vol.163, pp.116501-116501
- Publisher
- Elsevier
- DOI
- 10.1016/j.bone.2022.116501
- PMID
- 35872108
- ISSN
- 8756-3282
- eISSN
- 1873-2763
- Number of pages
- 15
- Grant note
- College of Dentistry, The Ohio State University; Ohio State University American Association of Orthodontists Foundation AG033714 / National Institute on Aging; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA)
- Language
- English
- Date published
- 10/01/2022
- Academic Unit
- Dental Research; Periodontics
- Record Identifier
- 9984367747902771
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