Journal article
Skin Injury Activates a Rapid TRPV1-Dependent Antiviral Protein Response
Journal of investigative dermatology, Vol.142(8), pp.2249-2259.e9
08/01/2022
DOI: 10.1016/j.jid.2021.11.041
PMCID: PMC9259761
PMID: 35007556
Abstract
The skin serves as the interface between the body and the environment and plays a fundamental role in innate antimicrobial host immunity. Antiviral proteins (AVPs) are part of the innate host defense system and provide protection against viral pathogens. How breach of the skin barrier influences innate AVP production remains largely unknown. In this study, we characterized the induction and regulation of AVPs after skin injury and identified a key role of TRPV1 in this process. Transcriptional and phenotypic profiling of cutaneous wounds revealed that skin injury induces high levels of AVPs in both mice and humans. Remarkably, pharmacologic and genetic ablation of TRPV1-mediated nociception abrogated the induction of AVPs, including Oas2, Oasl2, and Isg15 after skin injury in mice. Conversely, stimulation of TRPV1 nociceptors was sufficient to induce AVP production involving the CD301b(+) cells.IL-27.mediated signaling pathway. Using IL-27 receptor.knockout mice, we show that IL-27 signaling is required in the induction of AVPs after skin injury. Finally, loss of TRPV1 signaling leads to increased viral infectivity of herpes simplex virus. Together, our data indicate that TRPV1 signaling ensures skin antiviral competence on wounding.
Details
- Title: Subtitle
- Skin Injury Activates a Rapid TRPV1-Dependent Antiviral Protein Response
- Creators
- Vivian Lei - Duke UniversityChelsea Handfield - Duke UniversityJeffery T. Kwock - Duke UniversityStephen J. Kirchner - Duke UniversityMin Jin Lee - Duke UniversityMargaret Coates - Duke UniversityKaiyuan Wang - Duke UniversityQingjian Han - Duke UniversityZilong Wang - Duke UniversityJennifer G. Powers - Duke UniversitySarah Wolfe - Duke UniversityDavid L. Corcoran - Duke UniversityBrian Fanelli - Cosmos Corporation (United States)Manoj Dadlani - Cosmos Corporation (United States)Ru-Rong Ji - Duke UniversityJennifer Y. Zhang - Duke UniversityAmanda S. MacLeod - Duke University
- Resource Type
- Journal article
- Publication Details
- Journal of investigative dermatology, Vol.142(8), pp.2249-2259.e9
- DOI
- 10.1016/j.jid.2021.11.041
- PMID
- 35007556
- PMCID
- PMC9259761
- NLM abbreviation
- J Invest Dermatol
- ISSN
- 0022-202X
- eISSN
- 1523-1747
- Publisher
- Elsevier
- Number of pages
- 20
- Grant note
- R01 AI139207; R21AI139207; A031619 / National Institutes of Health, United States; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Silab company (Saint-Viance, France) partnership Eugene A. Stead Student Research Fellowship Duke PhysicianeScientist Strong Start Award Burroughs-Wellcome Student Research Fellowship Poindexter Fellowship Dermatology Foundation Award, United States
- Language
- English
- Date published
- 08/01/2022
- Academic Unit
- Dermatology
- Record Identifier
- 9984368219202771
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