Journal article
Skull bone regeneration in primates in response to basic fibroblast growth factor
Journal of neurosurgery, Vol.91(5), pp.851-856
11/1999
DOI: 10.3171/jns.1999.91.5.0851
PMID: 10541244
Abstract
Object.
The feasibility of using a biodegradable hydrogel incorporating basic fibroblast growth factor (bFGF) to induce bone regeneration at the site of a skull defect in monkeys was investigated.
Methods.
Basic fibroblast growth factor was incorporated into a bioabsorbable hydrogel, which was prepared through glutaraldehyde crosslinking of gelatin. Following treatment of monkey skull defects measuring 6 mm in diameter (six defects/experimental group) with gelatin hydrogel incorporating bFGF, skull bone regeneration was evaluated using soft x-ray studies, dual x-ray absorptometry, and histological examinations.
The water content of the hydrogels varied according to the glutaraldehyde concentration in the hydrogel preparation. Gelatin hydrogels incorporating 100 µg of bFGF significantly promoted bone regeneration and the skull defect was completely closed 21 weeks after implantation. This is in marked contrast with the effect of the same dose of bFGF in solution form. Bone mineral density (BMD) measured at the sites of skull defect was enhanced by the bFGF-incorporating hydrogels. The BMD enhancement was more prominent at lower water contents of hydrogel. Empty gelatin hydrogels neither induced nor interfered with skull bone regeneration.
Conclusions.
The findings of this study indicate that bFGF coupled with bioabsorbable hydrogel is a very promising tool to assist in the regrowth of bone at the site of a skull defect, which clinically has been recognized as almost impossible.
Details
- Title: Subtitle
- Skull bone regeneration in primates in response to basic fibroblast growth factor
- Creators
- Yasuhiko Tabata - Kyoto UniversityKeisuke YamadaLiu HongSusumu MiyamotoNobuo HashimotoYoshito Ikada
- Resource Type
- Journal article
- Publication Details
- Journal of neurosurgery, Vol.91(5), pp.851-856
- DOI
- 10.3171/jns.1999.91.5.0851
- PMID
- 10541244
- NLM abbreviation
- J Neurosurg
- ISSN
- 0022-3085
- eISSN
- 1933-0693
- Language
- English
- Date published
- 11/1999
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367729502771
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