Journal article
Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo
Development (Cambridge), Vol.145(10), pp.dev156604-dev156604
05/17/2018
DOI: 10.1242/dev.156604
PMCID: PMC6001370
PMID: 29632136
Abstract
Zebrafish germ plasm ribonucleoparticles (RNPs) become recruited to furrows of early zebrafish embryos through their association with astral microtubules ends. During the initiation of cytokinesis, microtubules are remodeled into a furrow microtubule array (FMA), which is thought to be analogous to the mammalian midbody involved in membrane abscission. During furrow maturation, RNPs and FMA tubules transition from their original distribution along the furrow to enrichments at the furrow distal ends, which facilitates germ plasm mass compaction. We show that
mutants exhibit reduced furrow-associated slow calcium waves (SCWs), caused at least in part by defective enrichment of calcium stores. RNP and FMA distal enrichment mirrors the medial-to-distal polarity of SCWs, and inhibition of calcium release or downstream mediators such as Calmodulin affects RNP and FMA distal enrichment. Blastomeres with reduced or lacking SCWs, such as early blastomeres in
mutants and wild-type blastomeres at later stages, exhibit medially bundling microtubules similar to midbodies in other cell types. Our data indicate that SCWs provide medial-to-distal directionality along the furrow to facilitate germ plasm RNP enrichment at the furrow ends.
Details
- Title: Subtitle
- Slow calcium waves mediate furrow microtubule reorganization and germ plasm compaction in the early zebrafish embryo
- Creators
- Celeste Eno - Laboratory of Genetics, University of Wisconsin - Madison, Madison, WI 53706, USATimothy Gomez - Department of Neuroscience, University of Wisconsin - Madison, Madison, WI 53705, USADiane C Slusarski - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAFrancisco Pelegri - Laboratory of Genetics, University of Wisconsin - Madison, Madison, WI 53706, USA fjpelegri@wisc.edu
- Resource Type
- Journal article
- Publication Details
- Development (Cambridge), Vol.145(10), pp.dev156604-dev156604
- Publisher
- England
- DOI
- 10.1242/dev.156604
- PMID
- 29632136
- PMCID
- PMC6001370
- ISSN
- 0950-1991
- eISSN
- 1477-9129
- Grant note
- F31 GM108449 / NIGMS NIH HHS R01 NS099405 / NINDS NIH HHS R56 GM065303 / NIGMS NIH HHS R01 CA112369 / NCI NIH HHS R01 GM065303 / NIGMS NIH HHS
- Language
- English
- Date published
- 05/17/2018
- Academic Unit
- Iowa Neuroscience Institute; Biology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984070448702771
Metrics
22 Record Views