Journal article
Smoking and Human Immunodeficiency Virus 1 Infection Promote Retention of CD8(+) T Cells in the Airway Mucosa
American journal of respiratory cell and molecular biology, Vol.65(5), pp.513-520
11/01/2021
DOI: 10.1165/rcmb.2021-0168OC
PMCID: PMC8641855
PMID: 34166603
Abstract
Smoking and human immunodeficiency virus 1 (HIV-1) infection are risk factors for chronic obstructive pulmonary dice-Aso (COPD), which is among the most common coniorbid conditions in people living with HIV-1. HIV-1 infection leads to persistent expansion of CD8(+) T cells, and CD8(+) T cell-mediated inflammation has been implicated in COPD pathogenesis. In this study, we investigated the effects of HIV-1 infection and smoking on T-cell dynamics in patients at risk of COPD. BAL fluid, endobronchial bnishings, and blood from HIV-1 infected and uninfected nonsmokers and smokers were analyzed by flow cytometry, and lungs were imaged by computed tomography. Chemokines were measured in BAL fluid, and CD8(+) T-cell chemotaxis in the presence of cigarette smoke extract was assessed in vitro. HIV-1 infection increased CD8(+) T cells in the BAL fluid, but this increase was abrogated by smoking Smokers had reduced BAL fluid concentrations of the T cell-recruiting chemokines CXCLIO and CCL5, and cigarette smoke extract inhibited CXCL10 and CCL5 production by macrophages and CD8(+) T-cell transmigration in vitro. In contrast to the T cells in RAL fluid, CD8(+) T cells in endobronchial brushings were increased in HIV-1-infected smokers, which was driven by an accumulation of effector memory T cells in the airway mucosa and an increase in tissue-resident memory T cells. Mucosal CD8(+) T-cell numbers inversely correlated with lung aeration, suggesting an association with inflammation and remodeling HIV-1 infection and smoking lead to retention of CD8(+) T cells within the airway mucosa.
Details
- Title: Subtitle
- Smoking and Human Immunodeficiency Virus 1 Infection Promote Retention of CD8(+) T Cells in the Airway Mucosa
- Creators
- Bjorn Corleis - Friedrich-Loeffler-InstitutJosalyn L. Cho - University of IowaSamantha J. Gates - Ragon Institute of MGH, MIT and HarvardAlice H. Linder - Ragon Institute of MGH, MIT and HarvardAmy Dickey - Ragon Institute of MGH, MIT and HarvardAntonella C. Lisanti-Park - Ragon Institute of MGH, MIT and HarvardAbigail E. Schiff - Ragon Institute of MGH, MIT and HarvardMusie Ghebremichael - Ragon Institute of MGH, MIT and HarvardPuja Kohli - Massachusetts General HospitalTilo Winkler - Massachusetts General HospitalR. Scott Harris - Massachusetts General HospitalBenjamin D. Medoff - Massachusetts General HospitalDouglas S. Kwon - Massachusetts General Hospital
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.65(5), pp.513-520
- Publisher
- Amer Thoracic Soc
- DOI
- 10.1165/rcmb.2021-0168OC
- PMID
- 34166603
- PMCID
- PMC8641855
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Number of pages
- 8
- Grant note
- 1U01HL121827-01; R01DA042685; 5 P30 AI060354010 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Harvard University Center for Acquired Immunodeficiency Syndrome Research
- Language
- English
- Date published
- 11/01/2021
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984359928302771
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