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Snf1-related kinase improves cardiac mitochondrial efficiency and decreases mitochondrial uncoupling
Journal article   Open access   Peer reviewed

Snf1-related kinase improves cardiac mitochondrial efficiency and decreases mitochondrial uncoupling

Amy K Rines, Hsiang-Chun Chang, Rongxue Wu, Tatsuya Sato, Arineh Khechaduri, Hidemichi Kouzu, Jason Shapiro, Meng Shang, Michael A Burke, Eltyeb Abdelwahid, …
Nature communications, Vol.8(1), pp.14095-14095
01/24/2017
DOI: 10.1038/ncomms14095
PMCID: PMC5286102
PMID: 28117339
url
https://doi.org/10.1038/ncomms14095View
Published (Version of record) Open Access

Abstract

Ischaemic heart disease limits oxygen and metabolic substrate availability to the heart, resulting in tissue death. Here, we demonstrate that the AMP-activated protein kinase (AMPK)-related protein Snf1-related kinase (SNRK) decreases cardiac metabolic substrate usage and mitochondrial uncoupling, and protects against ischaemia/reperfusion. Hearts from transgenic mice overexpressing SNRK have decreased glucose and palmitate metabolism and oxygen consumption, but maintained power and function. They also exhibit decreased uncoupling protein 3 (UCP3) and mitochondrial uncoupling. Conversely, Snrk knockout mouse hearts have increased glucose and palmitate oxidation and UCP3. SNRK knockdown in cardiac cells decreases mitochondrial efficiency, which is abolished with UCP3 knockdown. We show that Tribbles homologue 3 (Trib3) binds to SNRK, and downregulates UCP3 through PPARα. Finally, SNRK is increased in cardiomyopathy patients, and SNRK reduces infarct size after ischaemia/reperfusion. SNRK also decreases cardiac cell death in a UCP3-dependent manner. Our results suggest that SNRK improves cardiac mitochondrial efficiency and ischaemic protection.
Apoptosis Humans Male Gene Knockdown Techniques Myocardial Reperfusion Injury - pathology Myocardium - metabolism HEK293 Cells Protein-Serine-Threonine Kinases - antagonists & inhibitors Female Protein-Serine-Threonine Kinases - metabolism Repressor Proteins - metabolism Disease Models, Animal Cell Line Uncoupling Protein 3 - metabolism Isolated Heart Preparation Down-Regulation Mice, Inbred C57BL Cell Cycle Proteins - metabolism Protein-Serine-Threonine Kinases - genetics Mitochondria - metabolism Mice, Knockout Myocardium - cytology Animals Dogs Mice PPAR alpha - metabolism

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