Sodium Trimetaphosphate as a Novel Strategy for Matrix Metalloproteinase Inhibition and Dentin Remineralization

Rafael Simões Gonçalves; Polliana Mendes Candia Scaffa; Marina Ciccone Giacomini; Cristina de Mattos Pimenta Vidal; Heitor Marques Honório; Linda Wang

doi:10.1159/000484486

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Sodium Trimetaphosphate as a Novel Strategy for Matrix Metalloproteinase Inhibition and Dentin Remineralization

Journal article

Peer reviewed

Sodium Trimetaphosphate as a Novel Strategy for Matrix Metalloproteinase Inhibition and Dentin Remineralization

Rafael Simões Gonçalves, Polliana Mendes Candia Scaffa, Marina Ciccone Giacomini, Cristina de Mattos Pimenta Vidal, Heitor Marques Honório and Linda Wang

Caries research, Vol.52(3), pp.189-198

2018

DOI: 10.1159/000484486

PMID: 29342454

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Abstract

The effect of sodium trimetaphosphate (STMP) as an antiproteolytic and remineralizing agent on demineralized dentin was evaluated in vitro. The inhibitory potential of STMP at 0.5, 1.5, 3.5, and 5% against recombinant matrix metalloproteinases (MMPs) MMPs-2 and -9 was assessed by zymography. To investigate its remineralization potential, 40 bovine root specimens were obtained and subjected to a demineralization protocol to produce caries-like dentin lesions. After that, dentin surfaces were divided into 3 areas: (1) mineralized (no treatment); (2) demineralized; and (3) demineralized/treated with STMP and submitted to a pH-cycling associated or not with STMP (1.5, 3.5, or 5% STMP, 10 min of treatment). After that, superficial hardness (SH) and cross-sectional hardness (CSH) were determined. Polarized light microscopy (PLM) was used to qualitatively evaluate mineralization within the caries-like lesions. The zymographic analysis showed that STMP solution is a potent inhibitor of the gelatinolytic activity of MMPs-2 and -9 depending on the dose, since the lowest concentration (0.5%) partially inhibited the enzyme activity, while the higher concentrations completely inhibited enzyme activity. Regarding remineralization effect, only 1.5% STMP solution enhanced both the SH and CSH. PLM showed that the area treated with 1.5% STMP presented similar birefringence as mineralized sound dentin. In conclusion, 1.5% STMP solution is effective as an antiproteolytic agent against MMPs and promotes dentin remineralization.

Hardness - drug effects

Polyphosphates - pharmacology

Matrix Metalloproteinase Inhibitors - pharmacology

Tooth Remineralization - methods

Matrix Metalloproteinase 9 - metabolism

Dentin - drug effects

Matrix Metalloproteinase 2 - metabolism

Dentin - metabolism

Humans

In Vitro Techniques

Details

Title: Subtitle: Sodium Trimetaphosphate as a Novel Strategy for Matrix Metalloproteinase Inhibition and Dentin Remineralization
Creators: Rafael Simões Gonçalves - Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil
Polliana Mendes Candia Scaffa
Marina Ciccone Giacomini
Cristina de Mattos Pimenta Vidal
Heitor Marques Honório
Linda Wang
Resource Type: Journal article
Publication Details: Caries research, Vol.52(3), pp.189-198
DOI: 10.1159/000484486
PMID: 29342454
NLM abbreviation: Caries Res
ISSN: 0008-6568
eISSN: 1421-976X
Publisher: Switzerland
Language: English
Date published: 2018
Academic Unit: Operative Dentistry
Record Identifier: 9984066094602771

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