Journal article
Solitary Cholinergic Stimulation Induces Airway Hyperreactivity and Transcription of Distinct Pro-inflammatory Pathways
Lung, Vol.196(2), pp.219-229
04/2018
DOI: 10.1007/s00408-018-0091-0
PMCID: PMC5975219
PMID: 29380034
Abstract
Airway hyperreactivity is a hallmark feature of asthma and can be precipitated by airway insults, such as ozone exposure or viral infection. A proposed mechanism linking airway insults to airway hyperreactivity is augmented cholinergic transmission. In the current study, we tested the hypothesis that acute potentiation of cholinergic transmission is sufficient to induce airway hyperreactivity. We atomized the cholinergic agonist bethanechol to neonatal piglets and forty-eight hours later measured airway resistance. Bethanechol-treated piglets displayed increased airway resistance in response to intravenous methacholine compared to saline-treated controls. In the absence of an airway insult, we expected to find no evidence of airway inflammation; however, transcripts for several asthma-associated cytokines, including IL17A, IL1A, and IL8, were elevated in the tracheas of bethanechol-treated piglets. In the lungs, prior bethanechol treatment increased transcripts for IFNγ and its downstream target CXCL10. These findings suggest that augmented cholinergic transmission is sufficient to induce airway hyperreactivity, and raise the possibility that cholinergic-mediated regulation of pro-inflammatory pathways might contribute.
Details
- Title: Subtitle
- Solitary Cholinergic Stimulation Induces Airway Hyperreactivity and Transcription of Distinct Pro-inflammatory Pathways
- Creators
- Leah R Reznikov - University of FloridaDavid K Meyerholz - University of IowaShin-Ping Kuan - University of FloridaMaria V Guevara - University of FloridaKalina R Atanasova - University of FloridaMahmoud H Abou Alaiwa - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Lung, Vol.196(2), pp.219-229
- DOI
- 10.1007/s00408-018-0091-0
- PMID
- 29380034
- PMCID
- PMC5975219
- NLM abbreviation
- Lung
- ISSN
- 0341-2040
- eISSN
- 1432-1750
- Grant note
- P01 HL091842 / NHLBI NIH HHS R00 HL119560 / NHLBI NIH HHS K08 HL135433 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS K99 HL119560 / NHLBI NIH HHS OT2 OD023854 / NIH HHS
- Language
- English
- Date published
- 04/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pulmonary, Critical Care, and Occupational Medicine; Pathology; Internal Medicine
- Record Identifier
- 9984186571902771
Metrics
8 Record Views