Somatic cell apoptosis markers and pathways in human ejaculated sperm: potential utility as indicators of sperm quality

S L Taylor; S L Weng; P Fox; E H Duran; M S Morshedi; S Oehninger; S J Beebe

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Somatic cell apoptosis markers and pathways in human ejaculated sperm: potential utility as indicators of sperm quality

Journal article

Peer reviewed

Somatic cell apoptosis markers and pathways in human ejaculated sperm: potential utility as indicators of sperm quality

S L Taylor, S L Weng, P Fox, E H Duran, M S Morshedi, S Oehninger and S J Beebe

Molecular human reproduction, Vol.10(11), p.825

11/2004

PMID: 15465851

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Abstract

In this study we extended earlier work to determine whether sperm respond to somatic cell apoptotic stimuli and whether apoptotic phenotypes are significant indicators of human sperm quality. We evaluated ejaculated sperm from fertile donors and subfertile patients following purification of fractions of high and low motility. In unstimulated conditions, caspase enzymatic activity was higher in motile fractions from subfertile patients than in donors, and was higher in low motility fractions from both groups. Staurosporine, but not a Fas ligand or H2O2, significantly increased caspase activity, but only in high motility fractions. Procaspase-3, -7 and -9 and low levels of active caspase-3, -7 and -9 were identified by immunoblot analysis. Apoptosis-inducing factor (AIF) was present in all samples but poly ADP-ribose polymerase-1 (PARP-1) was not detected. Phosphatidylserine translocation was significantly increased only with H2O2 treatment. In ejaculates of both subfertile and fertile men, we demonstrated the presence and activation of several proteins that are key constituents of apoptosis-related pathways in somatic cells, which may serve as markers for sperm quality.

Apoptosis

Isoenzymes - analysis

Spermatozoa - chemistry

Apoptosis Inducing Factor

Humans

Male

Caspases - analysis

Spermatozoa - metabolism

Membrane Proteins - analysis

Ejaculation - physiology

Caspases - metabolism

Isoenzymes - metabolism

Flavoproteins - analysis

Adult

Membrane Proteins - metabolism

Infertility, Male - diagnosis

Biomarkers - metabolism

Flavoproteins - metabolism

Membrane Glycoproteins - pharmacology

Phosphatidylserines - metabolism

Biomarkers - analysis

Poly(ADP-ribose) Polymerases - analysis

Fas Ligand Protein

Sperm Motility - drug effects

Annexin A5 - metabolism

Poly(ADP-ribose) Polymerases - metabolism

Sperm Motility - physiology

Biological Transport - physiology

Spermatozoa - cytology

Staurosporine - pharmacology

Details

Title: Subtitle: Somatic cell apoptosis markers and pathways in human ejaculated sperm: potential utility as indicators of sperm quality
Creators: S L Taylor - Eastern Virginia Medical School
S L Weng - Eastern Virginia Medical School
P Fox
E H Duran - University of Iowa, Obstetrics and Gynecology
M S Morshedi
S Oehninger
S J Beebe
Resource Type: Journal article
Publication Details: Molecular human reproduction, Vol.10(11), p.825
Publisher: England
PMID: 15465851
ISSN: 1360-9947
eISSN: 1460-2407
Language: English
Date published: 11/2004
Academic Unit: Obstetrics and Gynecology
Record Identifier: 9983557123702771

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