Sparks and Puffs in Oligodendrocyte Progenitors: Cross Talk between Ryanodine Receptors and Inositol Trisphosphate Receptors

Laurel L Haak; Long-Sheng Song; Tadeusz F Molinski; Isaac N Pessah; Heping Cheng; James T Russell

doi:10.1523/JNEUROSCI.21-11-03860.2001

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Sparks and Puffs in Oligodendrocyte Progenitors: Cross Talk between Ryanodine Receptors and Inositol Trisphosphate Receptors

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Sparks and Puffs in Oligodendrocyte Progenitors: Cross Talk between Ryanodine Receptors and Inositol Trisphosphate Receptors

Laurel L Haak, Long-Sheng Song, Tadeusz F Molinski, Isaac N Pessah, Heping Cheng and James T Russell

The Journal of neuroscience, Vol.21(11), pp.3860-3870

06/01/2001

DOI: 10.1523/JNEUROSCI.21-11-03860.2001

PMCID: PMC6762718

PMID: 11356874

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Abstract

Investigating how calcium release from the endoplasmic reticulum (ER) is triggered and coordinated is crucial to our understanding of how oligodendrocyte progenitor cells (OPs) develop into myelinating cells. Sparks and puffs represent highly localized Ca 2+ release from the ER through ryanodine receptors (RyRs) and inositol trisphosphate receptors (IP 3 Rs), respectively. To study whether sparks or puffs trigger Ca 2+ waves in OPs, we performed rapid high-resolution line scan recordings in fluo-4-loaded OP processes. We found spontaneous and evoked sparks and puffs, and we have identified functional cross talk between IP 3 Rs and RyRs. Local events evoked using the IP 3 -linked agonist methacholine (MeCh) showed significantly different morphology compared with events evoked using the caffeine analog 3,7-dimethyl-1-propargylxanthine (DMPX). Pretreatment with MeCh potentiated DMPX-evoked events, whereas inhibition of RyRs potentiated events evoked by low concentrations of MeCh. Furthermore, activation of IP 3 Rs but not RyRs was critical for Ca 2+ wave initiation. Using immunocytochemistry, we show OPs express the specific Ca 2+ release channel subtypes RyR3 and IP 3 R2 in patches along OP processes. RyRs are coexpressed with IP 3 Rs in some patches, but IP 3 Rs are also found alone. This differential distribution pattern may underlie the differences in local and global Ca 2+ signals mediated by these two receptors. Thus, in OPs, interactions between IP 3 Rs and RyRs determine the spatial and temporal characteristics of calcium signaling, from microdomains to intracellular waves.

development

calcium

sparks

cross talk

SERCA

IP3 receptor

muscarinic receptor

Xestospongin C

wave

puffs

ARTICLE

oligodendrocyte progenitor

confocal microscopy

ryanodine receptor

Details

Title: Subtitle: Sparks and Puffs in Oligodendrocyte Progenitors: Cross Talk between Ryanodine Receptors and Inositol Trisphosphate Receptors
Creators: Laurel L Haak
Long-Sheng Song
Tadeusz F Molinski
Isaac N Pessah
Heping Cheng
James T Russell
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.21(11), pp.3860-3870
Publisher: Society for Neuroscience
DOI: 10.1523/JNEUROSCI.21-11-03860.2001
PMID: 11356874
PMCID: PMC6762718
ISSN: 0270-6474
eISSN: 1529-2401
Language: English
Date published: 06/01/2001
Academic Unit: Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984094662902771

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