Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels

Nicki K Baman; Galen B Schneider; Treniece L Terry; Rebecca Zaharias; Aliasger K Salem

doi:10.2147/nano.2006.1.2.213

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Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels

Journal article

Open access

Peer reviewed

Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels

Nicki K Baman, Galen B Schneider, Treniece L Terry, Rebecca Zaharias and Aliasger K Salem

International journal of nanomedicine, Vol.1(2), pp.213-217

2006

DOI: 10.2147/nano.2006.1.2.213

PMCID: PMC2426782

PMID: 17722538

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https://doi.org/10.2147/nano.2006.1.2.213View

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Abstract

We demonstrate spatial control over cell attachment on biodegradable surfaces by flowing cell adhesive poly (D-lysine) (PDL) in a trifluoroethanol (TFE)-water mixture through microfluidic channels placed on a biodegradable poly (lactic acid)-poly (ethylene glycol) (PLA-PEG) substrate. The partial solvent mixture swells the PLA-PEG within the confines of the microfluidic channels allowing PDL to diffuse on to the surface gel layer. When excess water is flowed through the channels substituting the TFE-water mixture, the swollen PLA surface collapses, entrapping PDL polymer. Results using preosteoblast human palatal mesenchymal cells (HEPM) indicate that this new procedure can be used for facile attachment of cells in localized regions. The PEG component of the PLA-PEG copolymer prevents cells from binding to the nonpatterned regions.

Flow Cytometry - instrumentation

Microfluidic Analytical Techniques - methods

Flow Cytometry - methods

Tissue Engineering - methods

Cell Culture Techniques - instrumentation

Solvents - chemistry

Humans

Osteoblasts - physiology

Cells, Cultured

Microfluidic Analytical Techniques - instrumentation

Coated Materials, Biocompatible - chemistry

Polylysine - chemistry

Cell Culture Techniques - methods

Tissue Engineering - instrumentation

Mesenchymal Stromal Cells - cytology

Cell Adhesion - physiology

Osteoblasts - cytology

Mesenchymal Stromal Cells - physiology

Details

Title: Subtitle: Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels
Creators: Nicki K Baman - Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA, USA
Galen B Schneider
Treniece L Terry
Rebecca Zaharias
Aliasger K Salem
Resource Type: Journal article
Publication Details: International journal of nanomedicine, Vol.1(2), pp.213-217
DOI: 10.2147/nano.2006.1.2.213
PMID: 17722538
PMCID: PMC2426782
NLM abbreviation: Int J Nanomedicine
ISSN: 1176-9114
eISSN: 1178-2013
Publisher: New Zealand
Grant note: P50 CA097274-04 / NCI NIH HHS P50 CA097274 / NCI NIH HHS
Language: English
Date published: 2006
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Dentistry Administration; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9983986439402771

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