Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors

Katherine Luby-Phelps; Joseph Fogerty; Sheila A Baker; Gregory J Pazour; Joseph C Besharse

doi:10.1016/j.visres.2007.08.022

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Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors

Journal article

Open access

Peer reviewed

Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors

Katherine Luby-Phelps, Joseph Fogerty, Sheila A Baker, Gregory J Pazour and Joseph C Besharse

Vision research (Oxford), Vol.48(3), pp.413-423

02/2008

DOI: 10.1016/j.visres.2007.08.022

PMCID: PMC2276311

PMID: 17931679

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Abstract

Intraflagellar transport (IFT) of a ∼17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP tagged IFT proteins. Endogenous IFT proteins are most highly concentrated within the inner segment, around the basal body, and within the outer segment IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus.

Rhodopsin

Intraflagellar transport

Microtubule

Connecting cilium

Kinesin

Photoreceptor

Details

Title: Subtitle: Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors
Creators: Katherine Luby-Phelps - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226-0509, USA
Joseph Fogerty - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226-0509, USA
Sheila A Baker - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226-0509, USA
Gregory J Pazour - Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
Joseph C Besharse - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226-0509, USA
Resource Type: Journal article
Publication Details: Vision research (Oxford), Vol.48(3), pp.413-423
DOI: 10.1016/j.visres.2007.08.022
PMID: 17931679
PMCID: PMC2276311
NLM abbreviation: Vision Res
ISSN: 0042-6989
eISSN: 1878-5646
Publisher: Elsevier Ltd
Language: English
Date published: 02/2008
Academic Unit: Iowa Neuroscience Institute; Biochemistry and Molecular Biology; University College Courses; Ophthalmology and Visual Sciences
Record Identifier: 9984025256602771

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