Spatial distribution of metals within the liver acinus and their perturbation by PCB126

William D Klaren; David Vine; Stefan Vogt; Larry W Robertson

doi:10.1007/s11356-017-0202-0

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Spatial distribution of metals within the liver acinus and their perturbation by PCB126

Journal article

Peer reviewed

Spatial distribution of metals within the liver acinus and their perturbation by PCB126

William D Klaren, David Vine, Stefan Vogt and Larry W Robertson

Environmental science and pollution research international, Vol.25(17), pp.16427-16433

06/2018

DOI: 10.1007/s11356-017-0202-0

PMCID: PMC5866157

PMID: 28940161

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Abstract

Animal studies show that exposure to the environmental pollutant 3,3',4,4',5-pentachlorobiphenyl (PCB126) causes alterations in hepatic metals as measured in acid-digested volume-adjusted tissue. These studies lack the detail of the spatial distribution within the liver. Here we use X-ray fluorescence microscopy (XFM) to assess the spatial distribution of trace elements within liver tissue. Liver samples from male Sprague Dawley rats, treated either with vehicle or PCB126, were formalin fixed and paraffin embedded. Serial sections were prepared for traditional H&E staining or placed on silicon nitride windows for XFM. With XFM, metal gradients between the portal triad and the central vein were seen, especially with copper and iron. These gradients change with exposure to PCB126, even reverse. This is the first report of how micronutrients vary spatially within the liver and how they change in response to toxicant exposure. In addition, high concentrations of zinc clusters were discovered in the extracellular space. PCB126 treatment did not affect their presence, but did alter their elemental makeup suggesting a more general biological function. Further work is needed to properly evaluate the gradients and their alterations as well as classify the zinc clusters to determine their role in liver function and zinc homeostasis.

Zinc - metabolism

Iron - chemistry

Liver

Micronutrients - metabolism

Trace Elements - chemistry

Homeostasis

Rats

Polychlorinated Biphenyls - metabolism

Zinc - chemistry

Iron - metabolism

Rats, Sprague-Dawley

Micronutrients - chemistry

Animals

Copper - chemistry

Polychlorinated Biphenyls - chemistry

Metals - chemistry

Copper - metabolism

ISRP Project 1 2015-2020

Synthesis Core

Argonne National Laboratories collaboration

KC Donnelly collaboration

Details

Title: Subtitle: Spatial distribution of metals within the liver acinus and their perturbation by PCB126
Creators: William D Klaren - Department of Occupational and Environmental Health, The University of Iowa, College of Public Health, 100 Oakdale Campus #219 IREH, Iowa City, IA, 52242-5000, USA
David Vine - Microscopy and X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Lemont, IL, USA
Stefan Vogt - Microscopy and X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Lemont, IL, USA
Larry W Robertson - Department of Occupational and Environmental Health, The University of Iowa, College of Public Health, 100 Oakdale Campus #219 IREH, Iowa City, IA, 52242-5000, USA. larry-robertson@uiowa.edu
Resource Type: Journal article
Publication Details: Environmental science and pollution research international, Vol.25(17), pp.16427-16433
DOI: 10.1007/s11356-017-0202-0
PMID: 28940161
PMCID: PMC5866157
NLM abbreviation: Environ Sci Pollut Res Int
ISSN: 0944-1344
eISSN: 1614-7499
Publisher: Germany
Grant note: P30 ES005605 / NIEHS NIH HHS P42 ES013661 / National Institute of Environmental Health Sciences P42 ES013661 / NIEHS NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 06/2018
Academic Unit: Occupational and Environmental Health; Iowa Superfund Research Program
Record Identifier: 9984002476502771

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