Journal article
Spatial modulation spectroscopy for imaging and quantitative analysis of single dye-doped organic nanoparticles inside cells
Nanoscale, Vol.7(21), pp.9779-9785
2015
DOI: 10.1039/C5NR01614B
PMCID: PMC4465101
PMID: 25964049
Abstract
Imaging of non-fluorescent nanoparticles in complex biological environments, such as the cell cytosol, is a challenging problem. For metal nanoparticles, Rayleigh scattering methods can be used, but for organic nanoparticles, such as dye-doped polymer beads or lipid nanoparticles, light scattering does not provide good contrast. In this paper, spatial modulation spectroscopy (SMS) is used to image single organic nanoparticles doped with non-fluorescent, near-IR croconaine dye. SMS is a quantitative imaging technique that yields the absolute extinction cross-section of the nanoparticles, which can be used to determine the number of dye molecules per particle. SMS images were recorded for particles within EMT-6 breast cancer cells. The measurements allowed mapping of the nanoparticle location and the amount of dye in a single cell. The results demonstrate how SMS can facilitate efforts to optimize dye-doped nanoparticles for effective photothermal therapy of cancer.
Details
- Title: Subtitle
- Spatial modulation spectroscopy for imaging and quantitative analysis of single dye-doped organic nanoparticles inside cells
- Creators
- Mary Sajini Devadas - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, USATuphan Devkota - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, USASamit Guha - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, USAScott K Shaw - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, USABradley D Smith - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, USAGregory V Hartland - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, USA
- Resource Type
- Journal article
- Publication Details
- Nanoscale, Vol.7(21), pp.9779-9785
- DOI
- 10.1039/C5NR01614B
- PMID
- 25964049
- PMCID
- PMC4465101
- NLM abbreviation
- Nanoscale
- ISSN
- 2040-3364
- eISSN
- 2040-3372
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R01GM059078, T32GM075762; DOI: 10.13039/100000165, name: Division of Chemistry, award: CHE-1110560; DOI: 10.13039/100000006, name: Office of Naval Research, award: N00014-12-1-1030
- Language
- English
- Date published
- 2015
- Academic Unit
- Chemistry
- Record Identifier
- 9984216594402771
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