Journal article
Spatial transcriptomics reveals unique gene expression changes in different brain regions after sleep deprivation
Nature communications, Vol.14(1), 7095
11/04/2023
DOI: 10.1038/s41467-023-42751-z
PMCID: PMC10625558
PMID: 37925446
Abstract
Sleep deprivation has far-reaching consequences on the brain and behavior, impacting memory, attention, and metabolism. Previous research has focused on gene expression changes in individual brain regions, such as the hippocampus or cortex. Therefore, it is unclear how uniformly or heterogeneously sleep loss affects the brain. Here, we use spatial transcriptomics to define the impact of a brief period of sleep deprivation across the brain in male mice. We find that sleep deprivation induced pronounced differences in gene expression across the brain, with the greatest changes in the hippocampus, neocortex, hypothalamus, and thalamus. Both the differentially expressed genes and the direction of regulation differed markedly across regions. Importantly, we developed bioinformatic tools to register tissue sections and gene expression data into a common anatomical space, allowing a brain-wide comparison of gene expression patterns between samples. Our results suggest that distinct molecular mechanisms acting in discrete brain regions underlie the biological effects of sleep deprivation.
Sleep deprivation impacts molecular changes across brain regions. Here, the authors utilize a spatial. transcriptomics approach to elucidate acute sleep deprivation-induced gene expression signature. across regions and subregions of the brain.
Details
- Title: Subtitle
- Spatial transcriptomics reveals unique gene expression changes in different brain regions after sleep deprivation
- Creators
- Yann Vanrobaeys - University of IowaZeru J. Peterson - University of IowaEmily. N. Walsh - University of IowaSnehajyoti Chatterjee - University of IowaLi-Chun Lin - University of IowaLisa C. Lyons - Florida State UniversityThomas Nickl-Jockschat - 169 Newton Road, 2312 Pappajohn Biomedical Discovery Building, Iowa City, IA USA 51 Newton Road, 2-417B Bowen Science Building, Iowa City, IA USA Iowa City, IA USATed Abel - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Nature communications, Vol.14(1), 7095
- DOI
- 10.1038/s41467-023-42751-z
- PMID
- 37925446
- PMCID
- PMC10625558
- NLM abbreviation
- Nat Commun
- ISSN
- 2041-1723
- eISSN
- 2041-1723
- Publisher
- Nature Publishing Group UK
- Grant note
- 1R01 AG 062398 / ;
- Language
- English
- Date published
- 11/04/2023
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984507158902771
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