Journal article
Spatiotemoporal regulation of Gβγ‐signaling and function by a novel Gβγ‐interacting protein, WDR26
The FASEB journal, Vol.25, pp.1011.5-1011.5
04/2011
DOI: 10.1096/fasebj.25.1_supplement.1011.5
Abstract
Heterotrimeric G protein βγ subunits play a critical role in many important cellular processes such as leukocyte migration. The essential role of Gβγ in directing leukocyte migration has been well established, but how it activates downstream effectors to generate a highly polarized intracellular signal that acts as an internal “compass” to drive directional cell migration remains unclear. Here, we characterized a novel interaction between Gβγ and WDR26, a WD40 protein that was identified from a yeast two‐hybrid screen and has unknown function. We showed that WDR26 associates with Gβγ following receptor activation, and is enriched at the leading edge of a polarized neutrophil. Inhibition of WDR26 expression by siRNAs abrogated directed leukocyte migration in vitro and T cell homing to lymphoid tissues in mice. Moreover, Gβγ‐mediated PI3K and PLCβ activation is alleviated by WDR26 suppression, suggesting that WDR26 is required for Gβγ signaling. Additional studies show that WDR26 binds and facilitates the activation of Gβγ effectors such as PLCβ2, probably by forming a large signaling complex containing both Gβγ and its effectors at the plasma membrane. Together, our data suggest that WDR26 is a novel interacting protein of Gβγ that may orchestrate spatiotemporal activation of downstream effectors of Gβγ required for precise control of directional cell migration.
Details
- Title: Subtitle
- Spatiotemoporal regulation of Gβγ‐signaling and function by a novel Gβγ‐interacting protein, WDR26
- Creators
- Songhai Chen - University of IowaZhizeng Sun - University of IowaXiaoyun Tang - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The FASEB journal, Vol.25, pp.1011.5-1011.5
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.25.1_supplement.1011.5
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 04/2011
- Academic Unit
- Internal Medicine; Neuroscience and Pharmacology; Iowa Neuroscience Institute
- Record Identifier
- 9984071731102771
Metrics
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