Spatiotemporal expression of MANF in the developing rat brain

Haiping Wang; Zunji Ke; Alexander Alimov; Mei Xu; Jacqueline A Frank; Shengyun Fang; Jia Luo

doi:10.1371/journal.pone.0090433

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Spatiotemporal expression of MANF in the developing rat brain

Journal article

Open access

Peer reviewed

Spatiotemporal expression of MANF in the developing rat brain

Haiping Wang, Zunji Ke, Alexander Alimov, Mei Xu, Jacqueline A Frank, Shengyun Fang and Jia Luo

PloS one, Vol.9(2), pp.e90433-e90433

2014

DOI: 10.1371/journal.pone.0090433

PMCID: PMC3938758

PMID: 24587361

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Abstract

Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an evolutionarily conserved neurotrophic factor which exhibited neuroprotective properties. Recent studies suggested that MANF may play a role in the neural development of Drosophila and zebra fishes. In this study, we investigated the spatiotemporal expression of MANF in the brain of postnatal and adult rats. MANF expression appeared wide spread and mainly localized in neurons. In the cerebral cortex, neurons in layer IV and VI displayed particularly strong MANF immunoreactivity. In the hippocampus, intensive MANF expression was observed throughout the subfields of Cornu Amonis (CA1, CA2, and CA3) and the granular layer of the dentate gyrus (DG). In the substantia nigra, high MANF expression was shown in the substantia nigra pars compacta (SNpc). In the thalamus, the anterodorsal thalamic nucleus (ADTN) exhibited the highest MANF immunoreactivity. In the hypothalamus, intensive MANF immunoreactivity was shown in the supraoptic nucleus (SON) and tuberomammillary nucleus (TMN). In the cerebellum, MANF was localized in the external germinal layer (EGL), Purkinje cell layer (PCL), internal granule layer (IGL) and the deep cerebellar nuclei (DCN). We examined the developmental expression of MANF on postnatal day (PD) 3, 5, 7, 9, 15, 21, 30 and adulthood. In general, the levels of MANF were high in the early PDs (PD3 and PD5), and declined gradually as the brain matured; MANF expression in the adult brain was the lowest among all time points examined. However, in some structures, such as PCL, IGL, SON, TMN and locus coeruleus (LC), high expression of MANF sustained throughout the postnatal period and persisted into adulthood. Our results indicated that MANF was developmentally regulated and may play a role in the maturation of the central nervous system (CNS).

Age Factors

Animals

Animals, Newborn

Cerebellum - growth & development

Cerebellum - metabolism

Cerebral Cortex - growth & development

Cerebral Cortex - metabolism

Female

Gene Expression Regulation, Developmental

Hippocampus - growth & development

Hippocampus - metabolism

Hypothalamus - growth & development

Hypothalamus - metabolism

Nerve Growth Factors - genetics

Nerve Growth Factors - metabolism

Organ Specificity

Rats

Rats, Sprague-Dawley

Spatio-Temporal Analysis

Substantia Nigra - growth & development

Substantia Nigra - metabolism

Thalamus - growth & development

Thalamus - metabolism

Details

Title: Subtitle: Spatiotemporal expression of MANF in the developing rat brain
Creators: Haiping Wang - University of Kentucky
Zunji Ke - Institute for Nutritional Sciences, SIBS, CAS, Shanghai, China.
Alexander Alimov - University of Kentucky
Mei Xu - University of Kentucky
Jacqueline A Frank - University of Kentucky
Shengyun Fang - University of Maryland, Baltimore
Jia Luo - University of Kentucky
Resource Type: Journal article
Publication Details: PloS one, Vol.9(2), pp.e90433-e90433
DOI: 10.1371/journal.pone.0090433
PMID: 24587361
PMCID: PMC3938758
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Grant note: I01 BX001721 / BLRD VA AA015407-09 / NIAAA NIH HHS R01 AA015407 / NIAAA NIH HHS
Language: English
Date published: 2014
Academic Unit: Pathology
Record Identifier: 9984201257002771

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