Species dependent expression of intestinal smooth muscle mechanosensitive sodium channels

P R Strege; A Mazzone; R E Kraichely; L Sha; A N Holm; Y Ou; I Lim; S J Gibbons; M G Sarr; G Farrugia

doi:10.1111/j.1365-2982.2006.00844.x

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Species dependent expression of intestinal smooth muscle mechanosensitive sodium channels

Journal article

Peer reviewed

Species dependent expression of intestinal smooth muscle mechanosensitive sodium channels

P R Strege, A Mazzone, R E Kraichely, L Sha, A N Holm, Y Ou, I Lim, S J Gibbons, M G Sarr and G Farrugia

Neurogastroenterology and motility, Vol.19(2), pp.135-143

02/2007

DOI: 10.1111/j.1365-2982.2006.00844.x

PMID: 17244168

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Abstract

A mechanosensitive Na(+) current carried by Na(v)1.5 is present in human intestinal circular smooth muscle and contributes to regulation of intestinal motor function. Expression of this channel in different species is unknown. Our aim was to determine if Na(+) currents and message for the alpha subunit of the Na(+) channel (SCN5A) are found in circular smooth muscle cells of human, dog, pig, mouse and guinea pig jejunum. Currents were recorded using patch clamp techniques. Message for SCN5A was investigated using laser capture microdissection and reverse transcription polymerase chain reaction (RT-PCR). Na(+) currents were identified consistently in human and dog smooth muscle cells; however, Na(+) current was not found in pig (0/20) or guinea pig smooth muscle cells (0/21) and found only one mouse cell (1/21). SCN5A mRNA was found in circular muscle of human, dog, and mouse, but not in pig or guinea pig, and not in mouse longitudinal or mucosal layers. In summary, SCN5A message is expressed in, and Na(+) current recorded from, circular muscle layer of human and dog but not from pig and guinea pig. These data show that there are species differences in expression of the SCN5A-encoded Na(v)1.5 channel, suggesting species-specific differences in the electrophysiological response to mechanical and depolarizing stimuli.

Guinea Pigs

Jejunum - physiology

Microdissection

Species Specificity

Humans

Mechanoreceptors - physiology

RNA, Messenger - analysis

Sodium Channels - physiology

Sodium - metabolism

Reverse Transcriptase Polymerase Chain Reaction

Patch-Clamp Techniques

Animals

NAV1.8 Voltage-Gated Sodium Channel

Lasers

Swine

Dogs

Electric Capacitance

Muscle, Smooth - physiology

Mice

Sodium Channels - genetics

NAV1.5 Voltage-Gated Sodium Channel

Details

Title: Subtitle: Species dependent expression of intestinal smooth muscle mechanosensitive sodium channels
Creators: P R Strege - Enteric NeuroScience Program, Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
A Mazzone
R E Kraichely
L Sha
A N Holm
Y Ou
I Lim
S J Gibbons
M G Sarr
G Farrugia
Resource Type: Journal article
Publication Details: Neurogastroenterology and motility, Vol.19(2), pp.135-143
DOI: 10.1111/j.1365-2982.2006.00844.x
PMID: 17244168
NLM abbreviation: Neurogastroenterol Motil
ISSN: 1350-1925
eISSN: 1365-2982
Grant note: DK 52766 / NIDDK NIH HHS DK 57061 / NIDDK NIH HHS
Language: English
Date published: 02/2007
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984094664202771

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