Journal article
Specific down-regulation of annexin II expression in human cells interferes with cell proliferation
Molecular and cellular biochemistry, Vol.199(1), pp.139-147
09/1999
DOI: 10.1023/A:1006942128672
PMID: 10544962
Abstract
The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and cell proliferation. Targeted disruption of the annexin II gene affects calcium signaling, tyrosine phosphorylation and apoptosis, indicating the important physiological role of this protein. We used a transient co-transfection assay to regulate annexin II expression in human HeLa, 293 and 293T cells, and measured the effects of annexin II down regulation on DNA synthesis and proliferation. Transfection of cells with an antisense annexin II vector results in inhibition of cell division and proliferation, with concomitant reduction in annexin II message and protein levels. Cellular DNA synthesis is significantly reduced in antisense transfected cells. Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation.
Details
- Title: Subtitle
- Specific down-regulation of annexin II expression in human cells interferes with cell proliferation
- Creators
- Yangping Chiang - Department of Biochemistry and Molecular Biology University of Nebraska Medical Center Omaha NE 68198 USAAngie Rizzino - Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center Omaha NE 68198 USAZita Sibenaller - Department of Biochemistry University of Iowa College of Medicie Iowa City IA 52242 USAMarc Wold - Department of Biochemistry University of Iowa College of Medicie Iowa City IA 52242 USAJamboor Vishwanatha - Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center Omaha NE 68198 USA
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biochemistry, Vol.199(1), pp.139-147
- Publisher
- Kluwer Academic Publishers; Dordrecht
- DOI
- 10.1023/A:1006942128672
- PMID
- 10544962
- ISSN
- 0300-8177
- eISSN
- 1573-4919
- Language
- English
- Date published
- 09/1999
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984025272202771
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