Spectral analysis by XANES reveals that GPNMB influences the chemical composition of intact melanosomes

Tamás Haraszti; Colleen M Trantow; Adam Hedberg-Buenz; Michael Grunze; Michael G Anderson

doi:10.1111/j.1755-148X.2010.00788.x

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Spectral analysis by XANES reveals that GPNMB influences the chemical composition of intact melanosomes

Journal article

Peer reviewed

Spectral analysis by XANES reveals that GPNMB influences the chemical composition of intact melanosomes

Tamás Haraszti, Colleen M Trantow, Adam Hedberg-Buenz, Michael Grunze and Michael G Anderson

Pigment cell and melanoma research, Vol.24(1), pp.187-196

02/2011

DOI: 10.1111/j.1755-148X.2010.00788.x

PMCID: PMC3021633

PMID: 21029394

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Abstract

GPNMB is a unique melanosomal protein. Unlike many melanosomal proteins, GPNMB has not been associated with any forms of albinism, and it is unclear whether GPNMB has any direct influence on melanosomes. Here, melanosomes from congenic strains of C57BL/6J mice mutant for Gpnmb are compared to strain-matched controls using standard transmission electron microscopy and synchrotron-based X-ray absorption near-edge structure analysis (XANES). Whereas electron microscopy did not detect any ultrastructural changes in melanosomes lacking functional GPNMB, XANES uncovered multiple spectral phenotypes. These results directly demonstrate that GPNMB influences the chemical composition of melanosomes and more broadly illustrate the potential for using genetic approaches in combination with nano-imaging technologies to study organelle biology.

Melanosomes - ultrastructure

Membrane Glycoproteins - metabolism

Oxidoreductases - metabolism

Oxidoreductases - genetics

Mutation - genetics

Melanosomes - chemistry

Membrane Glycoproteins - genetics

Phenotype

Animals

Eye Proteins - metabolism

Synchrotrons

Inheritance Patterns - genetics

Mice

Eye Proteins - genetics

X-Ray Absorption Spectroscopy - methods

Details

Title: Subtitle: Spectral analysis by XANES reveals that GPNMB influences the chemical composition of intact melanosomes
Creators: Tamás Haraszti - Institute of Physical Chemistry, University of Heidelberg, Heidelberg, Germany
Colleen M Trantow
Adam Hedberg-Buenz
Michael Grunze
Michael G Anderson
Resource Type: Journal article
Publication Details: Pigment cell and melanoma research, Vol.24(1), pp.187-196
DOI: 10.1111/j.1755-148X.2010.00788.x
PMID: 21029394
PMCID: PMC3021633
NLM abbreviation: Pigment Cell Melanoma Res
ISSN: 1755-1471
eISSN: 1755-148X
Publisher: England
Grant note: EY017673 / NEI NIH HHS CA086862-10 / NCI NIH HHS R03 AR055697 / NIAMS NIH HHS R03 AR055697-02 / NIAMS NIH HHS AR055697 / NIAMS NIH HHS P30 CA086862 / NCI NIH HHS R01 EY017673 / NEI NIH HHS R01 EY017673-03 / NEI NIH HHS
Language: English
Date published: 02/2011
Academic Unit: Molecular Physiology and Biophysics; Ophthalmology and Visual Sciences
Record Identifier: 9984025403402771

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