Journal article
Spent Culture Supernatant of Streptococcus gordonii Mitigates Inflammation of Human Periodontal Cells and Inhibits Proliferation of Pathogenic Oral Microbes
Journal of periodontology (1970), Vol.94(4), pp.575-585
04/2023
DOI: 10.1002/JPER.22-0333
PMCID: PMC10133002
PMID: 36369979
Appears in UI Libraries Support Open Access
Abstract
BACKGROUNDThe oral commensal bacterial species Streptococcus gordonii has been reported to regulate the inflammation of oral epithelial cells stimulated by the periodontal pathogen Porphyromonas gingivalis. This study investigated the activities of S. gordonii metabolites in S. gordonii spent culture supernatants (Sg-SCS) on periodontal-related bacterial growth and periodontitis-associated inflammatory cytokines. METHODSSg-SCS was collected from S. gordonii cultures grown in Dulbecco's Modified Eagle Medium (DMEM) and added to the growth media of representative health- and disease-related oral species: S. gordonii, Streptococcus sanguinis, Streptococcus mitis, Streptococcus oralis, P. gingivalis, Tannerella forsythia, and Treponema denticola. The Sg-SCS was also tested for its ability to regulate expression of proinflammatory cytokines by human macrophages, epithelial cells, and gingival fibroblasts upon stimulation with P. gingivalis-derived lipopolysaccharide (Pg-LPS). RESULTSSg-SCS significantly reduced transcript and protein levels of interleukin (IL)-1β, 6, and 8 induced by Pg-LPS stimulation in multiple types of periodontal cells. mRNA sequencing and bioinformatics analyses indicated that Sg-SCS significantly affects 10 inflammatory pathways. Additionally, Sg-SCS exhibited suppression of the growth of periodontal disease-related bacteria, including T. denticola and P. gingivalis, along with the primary plaque colonizing species S. oralis. At a low concentration, Sg-SCS also inhibits P. gingivalis adhesion. CONCLUSIONSThese results strongly suggest that S. gordonii-derived SCS contains metabolites that have anti-inflammatory properties and an ability to inhibit periodontitis-associated pathogenic bacteria. Further investigation will be needed to identify the individual metabolites within the Sg-SCS in order to develop a novel metabolite-based approach to treating and preventing periodontitis. This article is protected by copyright. All rights reserved.
Details
- Title: Subtitle
- Spent Culture Supernatant of Streptococcus gordonii Mitigates Inflammation of Human Periodontal Cells and Inhibits Proliferation of Pathogenic Oral Microbes
- Creators
- Yi Shu - University of IowaChawin Upara - University of IowaQiong Ding - University of IowaMin Zhu - University of IowaErliang Zeng - University of IowaJeffrey A Banas - University of IowaLiu Hong - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of periodontology (1970), Vol.94(4), pp.575-585
- DOI
- 10.1002/JPER.22-0333
- PMID
- 36369979
- PMCID
- PMC10133002
- NLM abbreviation
- J Periodontol
- eISSN
- 1943-3670
- Publisher
- Wiley
- Language
- English
- Electronic publication date
- 11/12/2022
- Date published
- 04/2023
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Prosthodontics; Pediatric Dentistry; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984317460102771
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