Spinal muscarinic receptors are activated during low or high frequency TENS-induced antihyperalgesia in rats

R RADHAKRISHNAN; K. A SLUKA

doi:10.1016/S0028-3908(03)00280-6

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Spinal muscarinic receptors are activated during low or high frequency TENS-induced antihyperalgesia in rats

Journal article

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Spinal muscarinic receptors are activated during low or high frequency TENS-induced antihyperalgesia in rats

R RADHAKRISHNAN and K. A SLUKA

Neuropharmacology, Vol.45(8), pp.1111-1119

2003

DOI: 10.1016/S0028-3908(03)00280-6

PMCID: PMC2746650

PMID: 14614954

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https://www.ncbi.nlm.nih.gov/pmc/articles/2746650View

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Abstract

Transcutaneous electrical nerve stimulation (TENS) is a non-pharmacological modality used clinically to relieve pain. Central involvement of serotonin and endogenous opioids are implicated in TENS-induced analgesia. Activation of spinal cholinergic receptors is antinociceptive and these receptors interact with opioid and serotonin receptors. In the current study, the possible involvement of spinal cholinergic receptors in TENS analgesia was investigated in rats. Hyperalgesia was induced by inflaming one knee joint with 3% kaolin-carrageenan and assessed by measuring paw withdrawal latency (PWL) to heat before and 4 h after injection. The non-selective nicotinic antagonist mecamylamine (50 microg), non-selective muscarinic antagonist atropine (30 microg) or one of the muscarinic subtype antagonists: pirenzepine (M1, 10 microg), methoctramine (M2, 10 microg), 4-DAMP (M3, 10 microg), or saline was administered intrathecally just prior to TENS treatment. Low or high frequency TENS was then applied to the inflamed knee and PWL was determined again. Atropine, pirenzepine and 4-DAMP significantly attenuated the antihyperalgesic effects of low and high frequency TENS while mecamylamine and methoctramine had no effects, compared to saline control. The results show that TENS-induced antihyperalgesia is mediated partially by activation of spinal muscarinic receptors but not spinal nicotinic receptors. Further, the results also indicate that spinal M1 and M3 muscarinic receptor subtypes mediate the muscarinic component of TENS antihyperalgesia.

Neuropharmacology

Fundamental and applied biological sciences. Psychology

Psychology. Psychoanalysis. Psychiatry

Psychology. Psychophysiology

Pharmacology. Drug treatments

Biological and medical sciences

Medical sciences

Details

Title: Subtitle: Spinal muscarinic receptors are activated during low or high frequency TENS-induced antihyperalgesia in rats
Creators: R RADHAKRISHNAN - Graduate Program in Physical Therapy and Rehabilitation Science, Pain Research Program, Neuroscience Graduate Program, 1-252 Medical Education Building, University of Iowa, Iowa City, IA 52242-1190, United States
K. A SLUKA - Graduate Program in Physical Therapy and Rehabilitation Science, Pain Research Program, Neuroscience Graduate Program, 1-252 Medical Education Building, University of Iowa, Iowa City, IA 52242-1190, United States
Resource Type: Journal article
Publication Details: Neuropharmacology, Vol.45(8), pp.1111-1119
DOI: 10.1016/S0028-3908(03)00280-6
PMID: 14614954
PMCID: PMC2746650
NLM abbreviation: Neuropharmacology
ISSN: 0028-3908
eISSN: 1873-7064
Publisher: Elsevier; Oxford
Language: English
Date published: 2003
Academic Unit: Iowa Neuroscience Institute; Nursing; Physical Therapy and Rehabilitation Science; Neuroscience and Pharmacology
Record Identifier: 9984040325402771

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