Spinal nociceptive transmission by mechanical stimulation of bone marrow

Takashi Ishida; Satoshi Tanaka; Takemi Sekiguchi; Daisuke Sugiyama; Mikito Kawamata

doi:10.1177/1744806916628773

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Spinal nociceptive transmission by mechanical stimulation of bone marrow

Journal article

Open access

Peer reviewed

Spinal nociceptive transmission by mechanical stimulation of bone marrow

Takashi Ishida, Satoshi Tanaka, Takemi Sekiguchi, Daisuke Sugiyama and Mikito Kawamata

Molecular pain, Vol.12, 1744806916628773

2016

DOI: 10.1177/1744806916628773

PMCID: PMC4994861

PMID: 27030710

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Abstract

Since bone marrow receives innervation from A-delta and C-fibers and since an increase in intramedullary pressure in bone marrow may induce acute pain in orthopedic patients during surgery and chronic pain in patients with bone marrow edema, skeletal pain may partly originate from bone marrow. Intraosseous lesions, such as osteomyelitis and bone cancer, are also known to produce cutaneous hypersensitivity, which might be referred pain from bone. However, little is known about pain perception in bone marrow and referred pain induced by bone disease. Thus, we carried out an in vivo electrophysiological study and behavioral study to determine whether increased intraosseous pressure of the femur induces acute pain and whether increased intraosseous pressure induces referred pain in the corresponding receptive fields of the skin. Intraosseous balloon inflation caused spontaneous pain-related behavior and mechanical hyperalgesia and allodynia in the lumbosacral region. Single neuronal activities of spinal dorsal horn neurons were extracellularly isolated, and then evoked responses to non-noxious and noxious cutaneous stimuli and intraosseous balloon inflation were recorded. Ninety-four spinal dorsal horn neurons, which had somatic receptive fields at the lower back and thigh, were obtained. Sixty-two percent of the wide-dynamic-range neurons (24/39) and 86% of the high-threshold neurons (12/14) responded to intraosseous balloon inflation, while none of the low-threshold neurons (0/41) responded to intraosseous balloon inflation. Spinally administered morphine (1 µg) abolished balloon inflation-induced spontaneous pain-related behavior and mechanical hyperalgesia in awake rats and also suppressed evoked activities of wide-dynamic-range neurons to noxious cutaneous stimulation and intraosseous balloon inflation. The results suggest that mechanical stimulation to bone marrow produces nociception, concomitantly producing its referred pain in the corresponding skin fields. These mechanisms might contribute to pain caused by skeletal diseases.

Animals

Bone Marrow - pathology

Catheterization

Evoked Potentials - drug effects

Femur - pathology

Ganglia, Spinal - drug effects

Ganglia, Spinal - pathology

Hyperalgesia - pathology

Hyperalgesia - physiopathology

Injections, Spinal

Male

Morphine - pharmacology

Naloxone - pharmacology

Nociception

Pain - etiology

Posterior Horn Cells - drug effects

Posterior Horn Cells - pathology

Rats, Sprague-Dawley

Sensation - drug effects

Spinal Cord - pathology

Spinal Cord - physiopathology

Stress, Mechanical

Details

Title: Subtitle: Spinal nociceptive transmission by mechanical stimulation of bone marrow
Creators: Takashi Ishida - Shinshu University
Satoshi Tanaka - Shinshu University
Takemi Sekiguchi - Shinshu University
Daisuke Sugiyama - Shinshu University
Mikito Kawamata - Shinshu University
Resource Type: Journal article
Publication Details: Molecular pain, Vol.12, 1744806916628773
DOI: 10.1177/1744806916628773
PMID: 27030710
PMCID: PMC4994861
NLM abbreviation: Mol Pain
ISSN: 1744-8069
eISSN: 1744-8069
Language: English
Date published: 2016
Academic Unit: Anesthesia
Record Identifier: 9984787459202771

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