Splenic transposition is superior to caudal shunt as a model of murine total hepatic ischemia

Tadashi Matsumoto; Philip A Efron; Hironori Tsujimoto; Sven K Tschoeke; Ricardo Ungaro; Shiro Fujita; David P Foley; Alan Hemming; Lyle L Moldawer

doi:10.1038/labinvest.3700210

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Splenic transposition is superior to caudal shunt as a model of murine total hepatic ischemia

Journal article

Open access

Peer reviewed

Splenic transposition is superior to caudal shunt as a model of murine total hepatic ischemia

Tadashi Matsumoto, Philip A Efron, Hironori Tsujimoto, Sven K Tschoeke, Ricardo Ungaro, Shiro Fujita, David P Foley, Alan Hemming and Lyle L Moldawer

Laboratory investigation, Vol.85(1), pp.90-98

01/2005

DOI: 10.1038/labinvest.3700210

PMID: 15558060

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Abstract

Murine total hepatic ischemia (THI) followed by reperfusion without shunting of the portal vein induces significant lethality in rodents due to intestinal congestion. Two methods have been promulgated to study THI and reperfusion in mice without intestinal congestion: subcutaneous splenic transposition which creates a portosystemic shunt via epigastric vessels, and a caudal shunt with 30% hepatectomy, which creates a portosystemic shunt via the small remnant of remaining caudal lobe. We compared outcome, inflammatory response and hepatic injury due to THI and reperfusion in these two models. Female C57BL/6 mice underwent ST, caudal shunt or no surgery prior to having 30 min of total hepatic ischemia followed by 60 min of reperfusion. Survival, surgical complications, serum AST/ALT and IL-6 were determined. Apoptotic and necrotic hepatocytes were identified by morphological criteria. Complication rates for the ST and caudal shunt procedures were 6.7 and 20%, respectively. Subsequent mortality rates following THI and 60 min reperfusion were 5.9 and 50% in mice with ST and caudal shunt, respectively. Both groups had elevated serum AST/ALT concentrations. However, in mice undergoing caudal shunt, AST/ALT levels were also significantly increased even without THI. The number of apoptotic hepatocytes after THI and reperfusion in mice following caudal shunt was significantly higher compared with those of ST (P<0.001). Both ST and caudal shunt can be used in models of THI and reperfusion to prevent significant lethality due to intestinal congestion. However, ST is a simple, safe and suitable model, whereas caudal shunt requires manipulation of the liver, and is associated with significant hepatic injury and morbidity.

Animals

Disease Models, Animal

Female

Hepatectomy

Intestines - blood supply

Intestines - pathology

Liver Diseases - mortality

Liver Diseases - physiopathology

Liver Diseases - surgery

Mice

Mice, Inbred C57BL

Portasystemic Shunt, Surgical

Reperfusion Injury - mortality

Reperfusion Injury - physiopathology

Reperfusion Injury - surgery

Spleen - physiopathology

Spleen - transplantation

Survival Rate

Time Factors

Details

Title: Subtitle: Splenic transposition is superior to caudal shunt as a model of murine total hepatic ischemia
Creators: Tadashi Matsumoto - University of Florida
Philip A Efron - University of Florida
Hironori Tsujimoto - University of Florida
Sven K Tschoeke - University of Florida
Ricardo Ungaro - University of Florida
Shiro Fujita - University of Florida
David P Foley - University of Florida
Alan Hemming - University of Florida
Lyle L Moldawer - University of Florida
Resource Type: Journal article
Publication Details: Laboratory investigation, Vol.85(1), pp.90-98
DOI: 10.1038/labinvest.3700210
PMID: 15558060
ISSN: 0023-6837
eISSN: 1530-0307
Grant note: R01 GM-63041-03 / NIGMS NIH HHS R37 GM-40561-15 / NIGMS NIH HHS
Language: English
Date published: 01/2005
Academic Unit: Surgery
Record Identifier: 9984322819702771

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