Splicing of two alternative exon pairs in β-tropomyosin pre-mRNA is independently controlled during myogenesis

YUNG-CHIH  WANG; P. A RUBENSTEIN

doi:10.1016/S0021-9258(19)49797-4

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Splicing of two alternative exon pairs in β-tropomyosin pre-mRNA is independently controlled during myogenesis

Journal article

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Splicing of two alternative exon pairs in β-tropomyosin pre-mRNA is independently controlled during myogenesis

YUNG-CHIH WANG and P. A RUBENSTEIN

The Journal of biological chemistry, Vol.267(17), pp.12004-12010

1992

DOI: 10.1016/S0021-9258(19)49797-4

PMID: 1601870

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Abstract

Two known tissue-specific tropomyosin (TM) isoforms are produced from the rodent beta-TM gene. Skeletal muscle beta-TM uses the alternative exons 6b and 9a and the exon 9a-associated poly(A) site. Fibroblast and smooth muscle TM-1 use exons 6a and 9b and the exon-9b associated poly(A) site. We have identified a new skeletal muscle beta-TM isoform, beta-TM2. beta-TM2 contains exon 6b (muscle) and exon 9b (nonmuscle). Full-length beta-TM2 cDNA clones were isolated from a cDNA library of mouse muscle BC3H1 cells. Its mRNA was also found in mouse skeletal muscle tissue but not in other tissues. beta-TM2 mRNA level and protein synthesis are differentiation-dependent, with a transient high level in the early stages of myogenesis both in BC3H1 cells and in mouse embryo limbs. Trace amounts of beta-TM3 mRNA, the other hybrid form (exons 6a + 9a), were found in less differentiated BC3H1 cells, mouse uterus, heart, and 3T3 fibroblasts but not skeletal muscle tissue. Thus, the selection of the two alternative exons appears to be controlled independently. Furthermore, during myogenesis, there is a sequential switch in the internal alternative exon, the terminal exon, and the poly(A) site from the nonmuscle to the muscle type.

Cell Physiology

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Molecular and cellular biology

Cell differentiation, maturation, development, hematopoiesis

Details

Title: Subtitle: Splicing of two alternative exon pairs in β-tropomyosin pre-mRNA is independently controlled during myogenesis
Creators: YUNG-CHIH WANG - Univ. Iowa coll. medicine, dep. biochemistry, Iowa City IA 52242, United States
P. A RUBENSTEIN - Univ. Iowa coll. medicine, dep. biochemistry, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.267(17), pp.12004-12010
DOI: 10.1016/S0021-9258(19)49797-4
PMID: 1601870
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology; Bethesda, MD
Language: English
Date published: 1992
Academic Unit: Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984025250202771

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