Spontaneous Ileitis and Post-Surgical Murine Models of Enteric Hyperoxaluria

Karim Jaber; Nadim Zaidan; Melody Ho; Xiaozhong Xiong; Rashmi Mishra; Ambika Nair; Arnav Mishra; Yi Chu; Mohamad Mokadem; Lama Nazzal

doi:10.1152/ajpgi.00043.2025

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Spontaneous Ileitis and Post-Surgical Murine Models of Enteric Hyperoxaluria

Journal article

Peer reviewed

Spontaneous Ileitis and Post-Surgical Murine Models of Enteric Hyperoxaluria

Karim Jaber, Nadim Zaidan, Melody Ho, Xiaozhong Xiong, Rashmi Mishra, Ambika Nair, Arnav Mishra, Yi Chu, Mohamad Mokadem and Lama Nazzal

American journal of physiology: Gastrointestinal and liver physiology, Vol.328(6), pp.G760-G773

06/01/2025

DOI: 10.1152/ajpgi.00043.2025

PMCID: PMC12119210

PMID: 40235154

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Abstract

Enteric hyperoxaluria, a risk factor for kidney stone disease, often arises from malabsorptive bariatric surgeries or inflammatory bowel diseases. Current murine models for studying this condition are limited, necessitating new approaches. This study aims to establish two novel and distinct mouse models to investigate enteric hyperoxaluria: one simulating Roux-en-Y gastric bypass surgery and the other Crohn's ileitis. In the first model, diet-induced obese C57BL/6J male mice underwent either sham or bypass surgery, followed by three weeks on a high-fat, oxalate-enriched diet. In the second model, SAMP1/YitFc and AKR mice were gradually introduced to high-fat diets, later supplemented with oxalate while reducing fat content. Samples of urine, blood, and feces were collected to assess oxalate, creatinine, and fecal lipid profiles. Results showed hyperoxaluria and increased stool fat content, indicating fat malabsorption, in both SAMP1 and bypass mice compared to controls. Kidney injury was also observed. These findings confirm the successful establishment of enteric hyperoxaluria in both models, highlighting the role of dietary oxalate, intestinal inflammation, and fat malabsorption in disease progression. These models provide valuable tools for exploring cellular and molecular mechanisms in enteric hyperoxaluria and may inform future therapeutic strategies.

Inflammatory Bowel Disease

Kidney Stones

Roux-en-Y gastric bypass

Fat malabsorption

Enteric Hyperoxaluria

Oxalate

Details

Title: Subtitle: Spontaneous Ileitis and Post-Surgical Murine Models of Enteric Hyperoxaluria
Creators: Karim Jaber - NYU Langone Health
Nadim Zaidan - NYU Langone Health
Melody Ho - NYU Langone Health
Xiaozhong Xiong - NYU Langone Health
Rashmi Mishra - NYU Langone Health
Ambika Nair - NYU Langone Health
Arnav Mishra - NYU Langone Health
Yi Chu - Veterans Affairs Health Care System, Iowa City, IA, USA
Mohamad Mokadem - Veterans Affairs Health Care System, Iowa City, IA, USA
Lama Nazzal - NYU Langone Health
Resource Type: Journal article
Publication Details: American journal of physiology: Gastrointestinal and liver physiology, Vol.328(6), pp.G760-G773
DOI: 10.1152/ajpgi.00043.2025
PMID: 40235154
PMCID: PMC12119210
NLM abbreviation: Am J Physiol Gastrointest Liver Physiol
ISSN: 0193-1857
eISSN: 1522-1547
Publisher: American Physiological Society
Grant note: 1R01DK129675 / HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Fraternal Order of Eagles (FOE) I01 BX004774-05 / U.S. Department of Veterans Affairs (VA) UI | Roy J. and Lucille A. Carver College of Medicine, University of Iowa (CCOM)
Language: English
Electronic publication date: 04/15/2025
Date published: 06/01/2025
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984810944102771

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