Journal article
Spontaneous Synaptic Transmission in Dissociated Meynert Neurons with the Attached Synaptic Boutons
Progress in neuro-psychopharmacology & biological psychiatry, Vol.17(3), pp.453-461
1993
DOI: 10.1016/0278-5846(93)90078-7
PMID: 8097334
Abstract
1.
1. Neurons acutely dissociated from the nucleus basalis of Meynert of adult rats showed spontaneous currents with rapid onset and slow decay under the whole-cell patch-clamp recording.
2.
2. The current had the reversal potential close to the theoretical Cl
− equilibrium potential (E
Cl) and was suppressed by 2 × 10
−6 M bicuculline and 3 x 10
−6 M strychnine.
3.
3. The spontaneous current was suppressed reversibly in the Ca
2+-free solution containing either EGTA or BAPTA (5mM). The enhancement of the frequency and amplitude of the current was observed with elevated extracellular K
+ concentration, which was also suppressed in the presence of the Ca
2+-free solution.
4.
4. Nicardipine, a blocker of voltage-dependent Ca
2+ channels, had an inhibitory effect as well.
5.
5. A selective GABA
B agonist, baclofen, blocked the spontaneous current, whereas a selective antagonist, phaclofen, potently enhanced the current.
6.
6. It is concluded that the observed spontaneous current was a spontaneous inhibitory postsynaptic current (IPSC) due to the release of GABA from the attached synaptic boutons. The release was suppressed by a GABA
B receptor activation, indicating that the GABA
B receptor regulates the release of GABA from nerve terminals and acts as a negative autoreceptor.
Details
- Title: Subtitle
- Spontaneous Synaptic Transmission in Dissociated Meynert Neurons with the Attached Synaptic Boutons
- Creators
- Nobutoshi HarataShinya UenoMitsutoshi MunakataNorio Akaike
- Resource Type
- Journal article
- Publication Details
- Progress in neuro-psychopharmacology & biological psychiatry, Vol.17(3), pp.453-461
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0278-5846(93)90078-7
- PMID
- 8097334
- ISSN
- 0278-5846
- eISSN
- 1878-4216
- Language
- English
- Date published
- 1993
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025333402771
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