Journal article
Sprayable ciprofloxacin-loaded poloxamer hydrogels for wound infection treatment
Journal of drug delivery science and technology, Vol.89, 105000
11/2023
DOI: 10.1016/j.jddst.2023.105000
PMCID: PMC10624398
PMID: 37928051
Abstract
Topical antimicrobial treatments for severe burns and chronic wounds provide effective treatment against infections, but cause pain and discomfort with application. This study aimed to develop an antimicrobial topical formulation comprising thermoreversible poloxamers (Pluronic F127 and F68) and a broad-spectrum antimicrobial agent (ciprofloxacin hydrochloride, CH), that could be sprayed to eliminate application pain while maintaining antimicrobial activity. Formulations were characterized to determine their sprayability under cold conditions, gelation temperature, final storage modulus at skin temperature, drug release profile, ex vivo permeation through impaired porcine skin, and inhibition against common bacterial pathogens that colonize wounds. All cold formulations were sprayable from simple hand-held, pump-action sprayers due to their low viscosity. Upon heating, 17 and 20% Pluronic F127 formulations produced hydrogels eight to ten degrees below skin temperature, independent of ciprofloxacin loading. Increasing concentrations of Pluronic F127 increased the final storage modulus and viscosity of the gels, while inclusion of Pluronic F68 reduced these properties, showing that hydrogel rheological properties at skin temperature can be tuned via choice of formulation. Drug release was directly correlated to the rheological properties, with stiffer gels resulting in a decrease in drug release rate. Overall, gels released about 65–90% of their load within 12 h. Ex vivo skin permeation demonstrated that drug was well retained in impaired porcine skin, which is desired to continuously treat bacteria localized to the wound. A well-diffusion assay indicated that the hydrogels had greater bacterial inhibition against Pseudomonas aeruginosa, Escherichia coli, and two strains of Staphylococcus aureus when compared to commercial controls. Overall, the results show the potential of CH-loaded poloxamer formulations as suitable sprayable topical dressings to deliver antimicrobials directly to wounds.
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Details
- Title: Subtitle
- Sprayable ciprofloxacin-loaded poloxamer hydrogels for wound infection treatment
- Creators
- Riannon Smith - University of IowaNicole Brogden - University of IowaJennifer Fiegel - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of drug delivery science and technology, Vol.89, 105000
- DOI
- 10.1016/j.jddst.2023.105000
- PMID
- 37928051
- PMCID
- PMC10624398
- NLM abbreviation
- J Drug Deliv Sci Technol
- ISSN
- 1773-2247
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R35GM124551, T32-GM008365; DOI: 10.13039/100012675, name: Center for Biocatalysis and Bioprocessing, University of Iowa
- Language
- English
- Date published
- 11/2023
- Academic Unit
- Dermatology; Pharmaceutical Sciences and Experimental Therapeutics; Chemical and Biochemical Engineering
- Record Identifier
- 9984473946302771
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