Spread of a neurotropic murine coronavirus into the CNS via the trigeminal and olfactory nerves

S Perlman; G Jacobsen; Adel  Afifi

doi:10.1016/0042-6822(89)90446-7

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Spread of a neurotropic murine coronavirus into the CNS via the trigeminal and olfactory nerves

Journal article

Open access

Peer reviewed

Spread of a neurotropic murine coronavirus into the CNS via the trigeminal and olfactory nerves

S Perlman, G Jacobsen and Adel Afifi

Virology, Vol.170(2), pp.556-560

06/1989

DOI: 10.1016/0042-6822(89)90446-7

PMCID: PMC7130896

PMID: 2543129

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Abstract

The route of entry into the central nervous system (CNS) of most neurtropic viruses has not been established. The coronavirus, mouse hepatitis virus strain JHM (MHV-JHM), causes acute encephalomyelitis and acute and chronic demyelinating diseases and is an important model system for virus-induced neurological disease. Suckling C57BL/6 mice infected intranasally with MHV-JHM develop either the acute encephalomyelitis or a late onset, symptomatic demyelinating encephalomyelitis, depending on whether they are nursed by unimmunized or immunized dams. Analysis by in situ hybridization was used to determine the route of entry of MHV-JHM into the CNS in these mice. At early times, viral RNA was detected only in the trigeminal and olfactory nerves and in their immediate connections in all mice. A few days later, MHV-JHM RNA was found throughout the brain in mice dying of the acute encephalomyelitis, but remained confined to the entry sites in mice which did not develop acute disease. These results suggest that MHV-JHM enters the CNS via an interneuronal route in all mice, but that the presence of maternal antibody prevents the dissemination of virus via extracellular fluid. In addition, MHV-JHM may establish low-level persistence in the trigeminal or olfactory nerve or in one of its connections in mice that do not develop acute encephalomyelitis.

Nucleic Acid Hybridization

Acute Disease

Immunity, Maternally-Acquired

Trigeminal Nerve - microbiology

Brain - microbiology

Olfactory Nerve - microbiology

Mice, Inbred C57BL

RNA, Viral - analysis

Murine hepatitis virus - physiology

Animals

Animals, Suckling

Spinal Cord - microbiology

Encephalomyelitis - immunology

Encephalomyelitis - microbiology

Mice

Murine hepatitis virus - genetics

Details

Title: Subtitle: Spread of a neurotropic murine coronavirus into the CNS via the trigeminal and olfactory nerves
Creators: S Perlman - Department of Pediatrics, University of Iowa, Iowa City 52242
G Jacobsen
Adel Afifi - University of Iowa, Stead Family Department of Pediatrics
Resource Type: Journal article
Publication Details: Virology, Vol.170(2), pp.556-560
DOI: 10.1016/0042-6822(89)90446-7
PMID: 2543129
PMCID: PMC7130896
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: United States
Grant note: R01 NS24401 / NINDS NIH HHS
Language: English
Date published: 06/1989
Academic Unit: Preventive and Community Dentistry; Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777469402771

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