Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast

Grzegorz Ira; Anna Malkova; Giordano Liberi; Marco Foiani; James E Haber

doi:10.1016/S0092-8674(03)00886-9

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Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast

Journal article

Open access

Peer reviewed

Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast

Grzegorz Ira, Anna Malkova, Giordano Liberi, Marco Foiani and James E Haber

Cell, Vol.115(4), pp.401-411

11/14/2003

DOI: 10.1016/S0092-8674(03)00886-9

PMCID: PMC4493758

PMID: 14622595

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Abstract

Very few gene conversions in mitotic cells are associated with crossovers, suggesting that these events are regulated. This may be important for the maintenance of genetic stability. We have analyzed the relationship between homologous recombination and crossing-over in haploid budding yeast and identified factors involved in the regulation of crossover outcomes. Gene conversions unaccompanied by a crossover appear 30 min before conversions accompanied by exchange, indicating that there are two different repair mechanisms in mitotic cells. Crossovers are rare (5%), but deleting the BLM/WRN homolog, SGS1, or the SRS2 helicase increases crossovers 2- to 3-fold. Overexpressing SRS2 nearly eliminates crossovers, whereas overexpression of RAD51 in srs2Delta cells almost completely eliminates the noncrossover recombination pathway. We suggest Sgs1 and its associated topoisomerase Top3 remove double Holliday junction intermediates from a crossover-producing repair pathway, thereby reducing crossovers. Srs2 promotes the noncrossover synthesis-dependent strand-annealing (SDSA) pathway, apparently by regulating Rad51 binding during strand exchange.

Crossing Over, Genetic

DNA Damage

DNA Helicases - genetics

DNA Helicases - metabolism

DNA Repair

DNA Topoisomerases, Type I - metabolism

DNA, Cruciform - chemistry

DNA, Cruciform - metabolism

DNA-Binding Proteins - genetics

DNA-Binding Proteins - metabolism

Gene Deletion

Mitosis - genetics

Rad51 Recombinase

RecQ Helicases

Saccharomyces cerevisiae - cytology

Saccharomyces cerevisiae - enzymology

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae Proteins - genetics

Saccharomyces cerevisiae Proteins - metabolism

Details

Title: Subtitle: Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast
Creators: Grzegorz Ira - Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
Anna Malkova
Giordano Liberi
Marco Foiani
James E Haber
Resource Type: Journal article
Publication Details: Cell, Vol.115(4), pp.401-411
DOI: 10.1016/S0092-8674(03)00886-9
PMID: 14622595
PMCID: PMC4493758
ISSN: 0092-8674
eISSN: 1097-4172
Grant note: R01 GM080600 / NIGMS NIH HHS GM61766 / NIGMS NIH HHS GM20056 / NIGMS NIH HHS R01 GM061766 / NIGMS NIH HHS R01 GM020056 / NIGMS NIH HHS R37 GM020056 / NIGMS NIH HHS
Language: English
Date published: 11/14/2003
Academic Unit: Biology
Record Identifier: 9984217525302771

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