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Stability and Robustness of a Generalized Pump-Leak Model for Epithelial Vesicles
Journal article   Open access   Peer reviewed

Stability and Robustness of a Generalized Pump-Leak Model for Epithelial Vesicles

Kerry Tarrant, Alan R Kay and Zahra Aminzare
Bulletin of mathematical biology, Vol.88(8), p.138
07/14/2026
DOI: 10.1007/s11538-026-01683-7
PMID: 42446753
Appears in  UI Libraries Support Open Access
url
https://doi.org/10.1007/s11538-026-01683-7View
Published (Version of record) Open Access

Abstract

Epithelial cells stabilize ion concentrations and volume through coordinated membrane pumps, ion channels, and paracellular pathways, which can be modeled by classical single-compartment pump-leak equations (PLEs). Many epithelial functions, however, depend on the interaction between a cell and an enclosed luminal space, a geometry that cannot be captured by classical PLEs. To address this, we develop a two-compartment model consisting of an intracellular compartment coupled to a luminal compartment through the apical membrane, with both compartments interfacing an infinite extracellular bath and connected to it through the basolateral membrane and a paracellular pathway. Building on the five-dimensional single-cell PLEs, we formulate a ten-dimensional PLE system for this geometry and derive analytical equilibria and steady-state formulas for both the passive system and the Na /K -ATPase (NKA) driven active system. We characterize how these states depend on physiologically relevant parameters, analyze local stability across wide parameter ranges, and apply global sensitivity and robustness methods to identify the principal determinants of ion and volume homeostasis. Our focus is on closed epithelial systems in which the lumen volume relaxes to a steady state, rather than on fluid-secreting epithelia with time-varying luminal volume. We quantify apical and basolateral membrane potentials at steady state; the basolateral potential varies widely across parameter regimes, whereas the apical potential remains comparatively small in magnitude. The model reveals fundamental differences between basolateral and apical placement of the NKA, including the onset of luminal volume expansion when apical potassium recycling is insufficient. More broadly, this framework provides a mathematically tractable and physiologically grounded foundation for studying epithelial transport and for predicting conditions under which pump localization and conductance changes lead to stable function or pathological lumen expansion.
 Pump-leak model Volume stabilization Sensitivity analysis Robustness UIOWA OA Agreement

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