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Stability of Mueller-Hinton agar supplemented with glucose and methylene blue for disk diffusion testing of fluconazole and voriconazole
Journal article   Open access   Peer reviewed

Stability of Mueller-Hinton agar supplemented with glucose and methylene blue for disk diffusion testing of fluconazole and voriconazole

M A Pfaller, L Boyken, S A Messer, R J Hollis and D J Diekema
Journal of clinical microbiology, Vol.42(3), pp.1288-1289
03/2004
DOI: 10.1128/JCM.42.3.1288-1289.2004
PMCID: PMC356905
PMID: 15004097
url
https://doi.org/10.1128/JCM.42.3.1288-1289.2004View
Published (Version of record) Open Access

Abstract

The shelf life of Mueller-Hinton agar supplemented with 2% glucose and methylene blue (0.5 micro g/ml) (MH-GMB) prepared in the laboratory to test disk diffusion of voriconazole and fluconazole was assessed using quality control (QC) strains of Candida albicans ATCC 90028, Candida krusei ATCC 6258, and Candida parapsilosis ATCC 22019. MH-GMB agar plates were prepared as described in National Committee for Clinical Laboratory Standards document M44-P, and isolates were tested using 25- micro g fluconazole disks and 1- micro g voriconazole disks over a 36-day period. Zone diameters for fluconazole and voriconazole varied by no more than 4 mm over the study period, and 95 to 100% of results were within the established QC limits for the strains tested. Prepared MH-GMB agar plates provide acceptable performance for disk diffusion testing for at least 30 days when stored at 5 degrees C.
 Fluconazole - pharmacology Agar Glucose Pyrimidines - pharmacology Candida - isolation & purification Triazoles - pharmacology Candida albicans - drug effects Microbial Sensitivity Tests - standards Culture Media Candida albicans - isolation & purification Voriconazole Methylene Blue Candida - drug effects Microbial Sensitivity Tests - methods Quality Control

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