Journal article
Stability of Peptide-Condensed Plasmid DNA Formulations
Journal of pharmaceutical sciences, Vol.87(6), pp.678-683
06/1998
DOI: 10.1021/js9800477
PMID: 9607943
Abstract
Low molecular weight homogeneous peptides were used to form peptide/DNA condensates. A peptide possessing 18 lysines was found to protect plasmid DNA from serum endonuclease and sonicative-induced degradation whereas a shorter peptide possessing 8 lysines dissociated in 0.1M sodium chloride and failed to protect DNA from enzymatic degradation. Peptide-condensed DNA showed no change in the ratio of supercoiled to circular DNA following 100W sonication for up to 60s and was able to transfect HepG2 cells with equivalent efficiency as untreated condensed plasmid DNA. Alternatively, uncondensed plasmid DNA was rapidly fragmented by sonication and serum endonucleases and resulted in negligible gene expression following condensation with peptide. Cationic lipid/DNA complexes were only partially effective at stabilizing DNA in serum compared to the complete stabilization afforded by peptide/DNA condensation. These results indicate that the stabilization afforded by condensation with a peptide protects DNA during formulation and preserves its structure in serum. These functions are important to achieve optimal gene expression from a nonviral gene delivery system.
Details
- Title: Subtitle
- Stability of Peptide-Condensed Plasmid DNA Formulations
- Creators
- Roger C AdamiWendy T CollardShamita A GuptaKai Y KwoKJeffrey BonadioKevin G Rice
- Resource Type
- Journal article
- Publication Details
- Journal of pharmaceutical sciences, Vol.87(6), pp.678-683
- DOI
- 10.1021/js9800477
- PMID
- 9607943
- NLM abbreviation
- J Pharm Sci
- ISSN
- 0022-3549
- eISSN
- 1520-6017
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 06/1998
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065692402771
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