Journal article
Stabilization of the reduced halocarbon-cytochrome P-450 complex of halothane by N-alkanes
Biochemical pharmacology, Vol.36(7), pp.1029-1034
1987
DOI: 10.1016/0006-2952(87)90410-2
PMID: 3566799
Abstract
Under anaerobic conditions, various halogenated compounds, when metabolized by cytochrome P-450, form complexes which are spectrally detectable. Previous studies have shown that halothane forms such a complex with cytochrome P-450, and the result is a strong absorption at 470 nm. Stabilization of this proposed intermediate carbanion complex has never been demonstrated in a biological system. Data are presented which show that several organic solvents (C
5-C
7N-alkanes) will stabilize the complex formed between halothane and cytochrome P-450. Stabilization allowed the decay of the complex to be studied, and it is demonstrated that the product of decay was chlorodifluoroethylene, which substantiates the hypothesis that the complex is a two electron-reduced carbanion. Carbon tetrachloride and benzyl bromide, which also produce spectrally visible intermediate complexes, were not stabilized by this treatment. Stabilization of the halothane complex in a biological system may facilitate studies to identify precisely the halothane-cytochrome P-450 complex and to clarify the mechanisms of halothane reduction by cytochrome P-450.
Details
- Title: Subtitle
- Stabilization of the reduced halocarbon-cytochrome P-450 complex of halothane by N-alkanes
- Creators
- Max T Baker - Department of Anesthesia, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.AJames N Bates - Department of Anesthesia, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.ARussell A Van Dyke - Department of Anesthesiology, Mayo Clinic, Rochester, MN 55905, U.S.A
- Resource Type
- Journal article
- Publication Details
- Biochemical pharmacology, Vol.36(7), pp.1029-1034
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0006-2952(87)90410-2
- PMID
- 3566799
- ISSN
- 0006-2952
- eISSN
- 1873-2968
- Language
- English
- Date published
- 1987
- Academic Unit
- Anesthesia
- Record Identifier
- 9984006312902771
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