Journal article
Stable H2O2-resistant variants of Chinese hamster fibroblasts demonstrate increases in catalase activity
Radiation research, Vol.114(1), pp.114-124
1988
DOI: 10.2307/3577149
PMID: 3353499
Abstract
Hydrogen peroxide (H2O2)-resistant variants of the Chinese hamster ovary HA-1 line have been derived by culturing cells in progressively higher concentrations of H2 O2 (>200 days, in 50-800 μM H2 O2). The H2O2-resistant phenotype has been stable for over 60 passages (240 days) following removal from the H2 O2 stress. The resistant cells demonstrate both increased capacity to deplete exogenously added H2 O2 from the growth medium and increased catalase activity. H2 O2 resistance correlates well with catalase activity. An increase in chromosome number occurred in the cells adapted to 200-800 μM H2 O2, but increases in aneuploidy and tetraploidy were not necessary for resistance. These results suggest that adaptation to chronic oxidative stress mediated by H2 O2 in mammalian cells is accompanied by a stable heritable change in expression of catalase activity.
Details
- Title: Subtitle
- Stable H2O2-resistant variants of Chinese hamster fibroblasts demonstrate increases in catalase activity
- Creators
- Douglas R Spitz - University of California, San FranciscoGloria C Li - Univ. California, radiation oncology res. lab., San Fransisco CA 94143, United StatesMichael L. McCormick - University of IowaYi Sun - University of IowaLarry W Oberley - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Radiation research, Vol.114(1), pp.114-124
- Publisher
- Radiation Research Society
- DOI
- 10.2307/3577149
- PMID
- 3353499
- ISSN
- 0033-7587
- eISSN
- 1938-5404
- Language
- English
- Date published
- 1988
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984312988602771
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