Journal article
Stage-specific oligonucleotide uptake in murine bone marrow B-cell precursors
Blood, Vol.84(11), pp.3660-3666
12/01/1994
DOI: 10.1182/blood.V84.11.3660.bloodjournal84113660
PMID: 7949121
Abstract
Fluorescein isothiocyanate (FITC)-conjugated phosphodiester and phosphorothioate oligonucleotides were used in four-color flow cytometry with murine bone marrow cells stained with monoclonal antibody specific for the differentiation markers B220, S7 (CD43), and BP-1 to show possible stage-specific oligonucleotide uptake. Relatively low uptake was observed among pre-Pro- and early Pro-B cells. Late Pro-B- and pre-B cells had increased oligonucleotide uptake, whereas B cells had a lower level. Cell membrane binding of oligonucleotides varied during B-cell differentiation in parallel with internalization, which was documented by confocal microscopy. An FITC-conjugated polyanionic dextran sulfate also showed differentiation-related B-cell association, suggesting the presence of cell membrane binding sites specific for polyanions as opposed to a unique feature of the DNA backbone. Interpretation of antisense experiments in murine bone marrow cells will need to account for the heterogeneous oligonucleotide uptake among differentiating B cells.
Details
- Title: Subtitle
- Stage-specific oligonucleotide uptake in murine bone marrow B-cell precursors
- Creators
- Qiuyan Zhao - Department of Internal Medicine, University of Iowa, Iowa CityThomas WaldschmidtEric FisherCharles J HerreraArthur M. Krieg
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.84(11), pp.3660-3666
- DOI
- 10.1182/blood.V84.11.3660.bloodjournal84113660
- PMID
- 7949121
- NLM abbreviation
- Blood
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Publisher
- United States
- Language
- English
- Date published
- 12/01/1994
- Academic Unit
- Pathology
- Record Identifier
- 9984047884602771
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