Journal article
Stamping an S on DMEK Donor Tissue to Prevent Upside-Down Grafts: Laboratory Validation and Detailed Preparation Technique Description
Cornea, Vol.34(9), pp.1175-1178
09/2015
DOI: 10.1097/ICO.0000000000000522
PMID: 26147839
Abstract
To report endothelial cell loss (ECL) caused by a novel S-stamp preparation technique for Descemet membrane endothelial keratoplasty (DMEK).
Six cadaveric human corneas were prepared for DMEK transplantation using a single standardized technique, including the application of a dry ink gentian violet S-stamp to the stromal side of Descemet membrane. Endothelial cell death was evaluated and quantified using computerized analysis of vital dye staining.
ECL caused by the S-stamp was 0.6% (range 0.1%-1.0%), which comprised less than one-tenth of the total ECL caused by our preparation of the DMEK graft from the start to finish, including recovery, prestripping, S-stamping, and trephination (13.7% total ECL, range 9.9%-17.6%).
Our novel S-stamp donor tissue preparation technique is intuitive to learn and holds the promise of preventing iatrogenic primary graft failure due to upside-down grafts without causing unacceptable increases in ECL.
Details
- Title: Subtitle
- Stamping an S on DMEK Donor Tissue to Prevent Upside-Down Grafts: Laboratory Validation and Detailed Preparation Technique Description
- Creators
- Peter B Veldman - Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, MA; †Cornea Service, Devers Eye Institute, Portland, OR; and ‡Lions VisionGift, Portland, ORPhilip K DyeJeffrey D HolimanZachary M MaykoChristopher S SálesMichael D StraikoChristopher G StoegerMark A Terry
- Resource Type
- Journal article
- Publication Details
- Cornea, Vol.34(9), pp.1175-1178
- DOI
- 10.1097/ICO.0000000000000522
- PMID
- 26147839
- ISSN
- 0277-3740
- eISSN
- 1536-4798
- Language
- English
- Date published
- 09/2015
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984111207002771
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