Journal article
Standardized Representation of Parts and Assembly for Build Planning
ACS synthetic biology, Vol.12(12), pp.3646-3655
12/15/2023
DOI: 10.1021/acssynbio.3c00418
PMID: 37956262
Abstract
The design and construction of genetic systems, in silico, in vitro, or in vivo, often involve the handling of various pieces of DNA that exist in different forms across an assembly process: as a standalone "part" sequence, as an insert into a carrier vector, as a digested fragment, etc. Communication about these different forms of a part and their relationships is often confusing, however, because of a lack of standardized terms. Here, we present a systematic terminology and an associated set of practices for representing genetic parts at various stages of design, synthesis, and assembly. These practices are intended to represent any of the wide array of approaches based on embedding parts in carrier vectors, such as BioBricks or Type IIS methods (e.g., GoldenGate, MoClo, GoldenBraid, and PhytoBricks), and have been successfully used as a basis for cross-institutional coordination and software tooling in the iGEM Engineering Committee.
Details
- Title: Subtitle
- Standardized Representation of Parts and Assembly for Build Planning
- Creators
- Jacob Beal - RTXVinoo Selvarajah - Workbar 45 Prospect St, Cambridge, MA 02139 USAGael Chambonnier - Massachusetts Institute of TechnologyTraci Haddock - Asimov Inc, Dept Biostat, Boston, MA 02215 USAAlejandro Vignoni - Universitat Politècnica de ValènciaGonzalo Vidal - Newcastle UniversityNicholas Roehner - RTX
- Resource Type
- Journal article
- Publication Details
- ACS synthetic biology, Vol.12(12), pp.3646-3655
- Publisher
- Amer Chemical Soc
- DOI
- 10.1021/acssynbio.3c00418
- PMID
- 37956262
- ISSN
- 2161-5063
- eISSN
- 2161-5063
- Number of pages
- 10
- Grant note
- FA8750-17-C-0184 / AFRL; United States Department of Defense; US Air Force Research Laboratory MCIN/AEI/10.13039/501100011033; PID2020-117271RB-C21 / Ministerio de Ciencia e Innovaci?n Newcastle University School of Computing
- Language
- English
- Date published
- 12/15/2023
- Academic Unit
- Electrical and Computer Engineering
- Record Identifier
- 9984627342602771
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