Journal article
Staphylococcal Exfoliative Toxins Cleave α- and β-Melanocyte-Stimulating Hormones
Infection and immunity, Vol.68(4), pp.2366-2368
Note
04/2000
DOI: 10.1128/IAI.68.4.2366-2368.2000
PMCID: PMC97430
PMID: 10722646
Abstract
The staphylococcal exfoliative toxins (ETs) A and B (ETA and ETB) are 27-kDa exotoxins produced by certain strains of
Staphylococcus aureus
and are the causative agents of staphylococcal scalded-skin syndrome. The crystal structures of the ETs strongly indicate that the proteins are members of the serine protease family of enzymes, although protease activity until now has not yet been conclusively demonstrated. Here, we show that the peptide β-melanocyte-stimulating hormone (β-MSH) is cleaved by ETA and that both ETA and ETB are capable of cleaving α-MSH. Both toxins exhibit cleavage at specific glutamic acid residues in MSH peptides. Moreover, biologically inactive mutants of ETA were incapable of cleaving β-MSH.
Details
- Title: Subtitle
- Staphylococcal Exfoliative Toxins Cleave α- and β-Melanocyte-Stimulating Hormones
- Creators
- James V Rago - University of Minnesota Medical School, Minneapolis, Minnesota 55455, and Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844Gregory M Vath - University of Minnesota Medical School, Minneapolis, Minnesota 55455, and Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844Timothy J Tripp - University of Minnesota Medical School, Minneapolis, Minnesota 55455, and Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844Gregory A Bohach - University of Minnesota Medical School, Minneapolis, Minnesota 55455, and Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844Douglas H Ohlendorf - University of Minnesota Medical School, Minneapolis, Minnesota 55455, and Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844Patrick M Schlievert - University of Minnesota Medical School, Minneapolis, Minnesota 55455, and Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.68(4), pp.2366-2368
- Series
- Note
- DOI
- 10.1128/IAI.68.4.2366-2368.2000
- PMID
- 10722646
- PMCID
- PMC97430
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 04/2000
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984002380602771
Metrics
9 Record Views