Starch Rich Chlorella vulgaris: High-Throughput Screening and Up-Scale for Tailored Biomass Production

Giorgia Carnovale; Filipa Rosa; Volha Shapaval; Simona Dzurendova; Achim Kohler; Trude Wicklund; Svein Jarle Horn; Maria J. Barbosa; Kari Skjanes

doi:10.3390/app11199025

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Starch Rich Chlorella vulgaris: High-Throughput Screening and Up-Scale for Tailored Biomass Production

Journal article

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Peer reviewed

Starch Rich Chlorella vulgaris: High-Throughput Screening and Up-Scale for Tailored Biomass Production

Giorgia Carnovale, Filipa Rosa, Volha Shapaval, Simona Dzurendova, Achim Kohler, Trude Wicklund, Svein Jarle Horn, Maria J. Barbosa and Kari Skjanes

Applied sciences, Vol.11(19), p.9025

10/01/2021

DOI: 10.3390/app11199025

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Abstract

The use of microalgal starch has been studied in biorefinery frameworks to produce bioethanol or bioplastics, however, these products are currently not economically viable. Using starch-rich biomass as an ingredient in food applications is a novel way to create more value while expanding the product portfolio of the microalgal industry. Optimization of starch production in the food-approved species Chlorella vulgaris was the main objective of this study. High-throughput screening of biomass composition in response to multiple stressors was performed with FTIR spectroscopy. Nitrogen starvation was identified as an important factor for starch accumulation. Moreover, further studies were performed to assess the role of light distribution, investigating the role of photon supply rates in flat panel photobioreactors. Starch-rich biomass with up to 30% starch was achieved in cultures with low inoculation density (0.1 g L-1) and high irradiation (1800 mu mol m(-2) s(-1)). A final large-scale experiment was performed in 25 L tubular reactors, achieving a maximum of 44% starch in the biomass after 12 h in nitrogen starved conditions.

Chemistry

Chemistry, Multidisciplinary

Engineering

Engineering, Multidisciplinary

Materials Science

Materials Science, Multidisciplinary

Physical Sciences

Physics

Physics, Applied

Science & Technology

Technology

Details

Title: Subtitle: Starch Rich Chlorella vulgaris: High-Throughput Screening and Up-Scale for Tailored Biomass Production
Creators: Giorgia Carnovale - Norwegian University of Life Sciences
Filipa Rosa - Norwegian Institute of Bioeconomy Research
Volha Shapaval - Norwegian University of Life Sciences
Simona Dzurendova - Norwegian University of Life Sciences
Achim Kohler - Norwegian University of Life Sciences
Trude Wicklund - Norwegian University of Life Sciences
Svein Jarle Horn - Norwegian University of Life Sciences
Maria J. Barbosa - Wageningen Univ, Bioproc Engn, AlgaePARC, POB 16, NL-6700 AA Wageningen, Netherlands
Kari Skjanes - Norwegian Institute of Bioeconomy Research
Resource Type: Journal article
Publication Details: Applied sciences, Vol.11(19), p.9025
DOI: 10.3390/app11199025
ISSN: 2076-3417
eISSN: 2076-3417
Publisher: Mdpi
Number of pages: 14
Grant note: 82845 / NordForsk (NCoE Programme "NordAqua") 267872 / Research Council Norway (BIONAER programme, "ALGAE TO FUTURE)
Language: English
Date published: 10/01/2021
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984398021802771

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