Journal article
Stem cells in the lung
Methods in enzymology, Vol.419, pp.285-321
2006
DOI: 10.1016/S0076-6879(06)19012-6
PMCID: PMC1803078
PMID: 17141060
Abstract
The lung is composed of two major anatomically distinct regions-the conducting airways and gas-exchanging airspaces. From a cell biology standpoint, the conducting airways can be further divided into two major compartments, the tracheobronchial and bronchiolar airways, while the alveolar regions of the lung make up the gas-exchanging airspaces. Each of these regions consists of distinct epithelial cell types with unique cellular physiologies and stem cell compartments. This chapter focuses on model systems with which to study stem cells in the adult tracheobronchial airways, also referred to as the proximal airway of the lung. Important in such models is an appreciation for the diversity of stem cell niches in the conducting airways that provide localized environmental signals to both maintain and mobilize stem cells in the setting of airway injury and normal cellular turnover. Because cellular turnover in airways is relatively slow, methods for analysis of stem cells in vivo have required prior injury to the lung. In contrast, ex vivo and in vitro models for analysis of airway stem cells have used genetic markers to track lineage relationships together with reconstitution systems that mimic airway biology. Over the past decades, several widely acceptable methods have been developed and used in the characterization of adult airway stem/progenitor cells. These include localization of label-retaining cells (LRCs), retroviral tagging of epithelial cells seeded into xenografts, air-liquid interface cultures to track clonal proliferative potential, and multiple transgenic mouse models. This chapter reviews the biologic context and use of these models while providing detailed methods for several of the more broadly useful models for studying adult airway stem/progenitor cell types.
Details
- Title: Subtitle
- Stem cells in the lung
- Creators
- Xiaoming Liu - Center for Gene Therapy, University of Iowa, Iowa City, IA, USARyan R DriskellJohn F Engelhardt
- Resource Type
- Journal article
- Publication Details
- Methods in enzymology, Vol.419, pp.285-321
- DOI
- 10.1016/S0076-6879(06)19012-6
- PMID
- 17141060
- PMCID
- PMC1803078
- NLM abbreviation
- Methods Enzymol
- ISSN
- 0076-6879
- eISSN
- 1557-7988
- Publisher
- United States
- Grant note
- R37 DK047967 / NIDDK NIH HHS R01 DK047967 / NIDDK NIH HHS P30 DK054759 / NIDDK NIH HHS DK47967 / NIDDK NIH HHS
- Language
- English
- Date published
- 2006
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025460202771
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