Journal article
Stem cells rejuvenate radiation-impaired vasculogenesis in murine distraction osteogenesis
Plastic and reconstructive surgery (1963), Vol.135(3), pp.799-806
03/01/2015
DOI: 10.1097/PRS.0000000000001024
PMCID: PMC4608845
PMID: 25415276
Abstract
BACKGROUNDRadiotherapy is known to be detrimental to bone and soft-tissue repair. Bone marrow stromal cells have been shown to enhance bone regeneration during distraction osteogenesis following radiation therapy. The authors posit that transplanted bone marrow stromal cells will significantly augment the mandibular vascularity devastated by radiation therapy. METHODSNineteen male Lewis rats were split randomly into three groups: distraction osteogenesis only (n = 5), radiation therapy plus distraction osteogenesis (n = 7), and radiation therapy plus distraction osteogenesis with intraoperative placement of 2 million bone marrow stromal cells (n = 7). A mandibular osteotomy was performed, and an external fixator device was installed. From postoperative days 4 through 12, rats underwent a gradual 5.1-mm distraction followed by a 28-day consolidation period. On postoperative day 40, Microfil was perfused into the vasculature and imaging commenced. Vascular radiomorphometric values were calculated for regions of interest. An analysis of variance with post hoc Tukey or Games-Howell tests was used, dependent on data homogeneity. RESULTSStereologic analysis indicated significant remediation in vasculature in the bone marrow stromal cell group compared with the radiation therapy/distraction osteogenesis group. Each of five metrics idicated significant improvements from radiation therapy/distraction osteogenesis to the bone marrow stromal cell group, with no difference between the bone marrow stromal cell group and the distraction osteogenesis group. CONCLUSIONSBone marrow stromal cells used together with distraction osteogenesis can rejuvenate radiation-impaired vasculogenesis in the mandible, reversing radiation therapy-induced isotropy and creating a robust vascular network. Bone marrow stromal cells may offer clinicians an alternative reconstructive modality that could improve the lifestyle of patients with hypovascular bone.
Details
- Title: Subtitle
- Stem cells rejuvenate radiation-impaired vasculogenesis in murine distraction osteogenesis
- Creators
- Sagar S Deshpande - University of MichiganKathleen K Gallagher - Michigan MedicineAlexis Donneys - University of MichiganNoah S Nelson - University of MichiganNicholas P Guys - Medical University of South CarolinaPeter A Felice - University of MichiganErin E Page - University of MichiganHongli Sun - University of MichiganPaul H Krebsbach - Biomedical Engineering - Medical SchoolSteven R Buchman - Neurosurgery
- Resource Type
- Journal article
- Publication Details
- Plastic and reconstructive surgery (1963), Vol.135(3), pp.799-806
- DOI
- 10.1097/PRS.0000000000001024
- PMID
- 25415276
- PMCID
- PMC4608845
- NLM abbreviation
- Plast Reconstr Surg
- ISSN
- 0032-1052
- eISSN
- 1529-4242
- Language
- English
- Date published
- 03/01/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery
- Record Identifier
- 9984367730502771
Metrics
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