Journal article
Stereoselective actions of S-nitrosocysteine in central nervous system of conscious rats
American journal of physiology. Heart and circulatory physiology, Vol.272(5), pp.H2361-H2368
05/01/1997
DOI: 10.1152/ajpheart.1997.272.5.H2361
PMID: 9176306
Abstract
This study examined whether the stereoselective actions of S-nitrosocysteine (SNC) in the central nervous system involves the activation of stereoselective SNC recognition sites. We examined the effects produced by intracerebroventricular injection of the L- and D-isomers of SNC (L- and D-SNC) on mean arterial blood pressure, heart rate, and vascular resistances in conscious rats. We also examined the hemodynamic effects produced by intracerebroventricular injections of 1) L-cystine, the major non-nitric oxide (NO) decomposition product of L-SNC, 2) the parent thiols L- and D-cysteine, and 3) the bulky S-nitrosothiol L-S-nitroso-gamma-glutamylcysteinylglycine [L-S-nitrosoglutathione, (L-SNOG)]. Finally, we examined the decomposition of L- and D-SNC and L-SNOG to NO on their addition to brain homogenates. The intracerebroventricular injection of L-SNC (250-1,000 nmol) produced falls in mean arterial pressure, increases in heart rate, and a dose-dependent pattern of changes in hindquarter, renal, and mesenteric vascular resistances. The intracerebroventricular injections of D-SNC, L-cystine, and L-SNOG produced only minor effects. The intracerebroventricular injection of L-cysteine produced pressor responses and tachycardia, whereas D-cysteine was inactive. L- and D-SNC decomposed equally to NO on addition to brain homogenates. L-SNOG decomposed to similar amounts of NO as L- and D-SNC. These results suggest that SNC may activate stereoselective SNC recognition sites on brain neurons and that S-nitrosothiols of substantially different structure do not stimulate these sites. These recognition sites may be stereoselective membrane-bound receptors for which L-SNC is the unique ligand.
Details
- Title: Subtitle
- Stereoselective actions of S-nitrosocysteine in central nervous system of conscious rats
- Creators
- Robin L Davisson - Cardiovascular Center, University of Iowa, Iowa City 52242, USAMark D Travis - Cardiovascular Center, University of Iowa, Iowa City 52242, USAJames N Bates - Cardiovascular Center, University of Iowa, Iowa City 52242, USAA Kim Johnson - Cardiovascular Center, University of Iowa, Iowa City 52242, USAStephen J Lewis - Cardiovascular Center, University of Iowa, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.272(5), pp.H2361-H2368
- DOI
- 10.1152/ajpheart.1997.272.5.H2361
- PMID
- 9176306
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 05/01/1997
- Academic Unit
- Psychological and Brain Sciences; Anesthesia; Neuroscience and Pharmacology; Health and Human Physiology
- Record Identifier
- 9984006440302771
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