Journal article
Stimulation of lumbar sympathetic nerves may produce hindlimb vasodilation via the release of pre-formed stores of nitrosyl factors
Neuroscience, Vol.72(4), pp.881-887
1996
DOI: 10.1016/0306-4522(96)00090-5
PMID: 8735216
Abstract
The physiological activation of lumbar sympathetic nerves by air-jet stress produces a hindlimb vasodilation in conscious rats. Although the nitric oxide synthase inhibitor N
G-vitro-
l-arginine methyl ester markedly reduces the duration of this air-jet stress-induced vasodilation, it does not prevent the initial fall in resistance. These data suggest that the vasodilation is initiated by the release of an as yet unidentified factor, whereas the vasodilation is sustained by the release of nitric oxide or newly synthesized nitrosyl factors such as S-nitrosothiols. At present, the possibility that neurogenic vasodilation may be initiated by the release of pre-formed pools of nitrosyl factors from storage sites within the hindlimb vasculature has not been addressed. We reasoned that if nitrosyl factors do exist in storage pools, then we should be able to demonstrate a “use-dependent” loss of vasodilation after nitric oxide synthesis inhibition which would be the result of a gradual depletion of the releasable pools of these nitrosyl factors. In the present study, we examined the effects of repeated episodes of direct electrical stimulation of the lumbar sympathetic chain on ipsilateral hindlimb vascular resistance in pentobarbital-anesthetized rats prior to and following administration of the nitric oxide synthesis inhibitors N
G-vitro-
l-arginine methyl ester (10, 25 or 100pmol/kg i.v.) or N
G-vitro-
l-arginine (50 umol/kg i.v.). Three episodes of electrical stimulation at 3.2 ± 0.4 V (20 Hz, 5 ms duration, 5 ms delay for 10 s given 5 min apart) produced pronounced and reproducible reductions in hindlimb vascular resistance
Details
- Title: Subtitle
- Stimulation of lumbar sympathetic nerves may produce hindlimb vasodilation via the release of pre-formed stores of nitrosyl factors
- Creators
- R.L Davisson - The Cardiovascular Center and the Department of Pharmacology, The University of Iowa, Iowa City, IA 52242, U.S.AR.A Shaffer - The Cardiovascular Center and the Department of Pharmacology, The University of Iowa, Iowa City, IA 52242, U.S.AA.K Johnson - Department of Psychology, The University of Iowa, Iowa City, IA 52242, U.S.AS.J Lewis - The Cardiovascular Center and the Department of Pharmacology, The University of Iowa, Iowa City, IA 52242, U.S.A
- Resource Type
- Journal article
- Publication Details
- Neuroscience, Vol.72(4), pp.881-887
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/0306-4522(96)00090-5
- PMID
- 8735216
- ISSN
- 0306-4522
- eISSN
- 1873-7544
- Language
- English
- Date published
- 1996
- Academic Unit
- Psychological and Brain Sciences; Neuroscience and Pharmacology; Health and Human Physiology
- Record Identifier
- 9984213409502771
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