Stimulation of rat granulosa cell progesterone production but not other differentiated functions by a splenocyte-derived factor

J M Ness; B G Kasson

doi:10.1530/jrf.0.1050125

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Stimulation of rat granulosa cell progesterone production but not other differentiated functions by a splenocyte-derived factor

Journal article

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Stimulation of rat granulosa cell progesterone production but not other differentiated functions by a splenocyte-derived factor

J M Ness and B G Kasson

Journal of reproduction & fertility, Vol.105(1), pp.125-133

09/1995

DOI: 10.1530/jrf.0.1050125

PMID: 7490703

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Abstract

Concanavalin A-stimulated splenocytes secrete a factor that stimulates progesterone production in cultured rat granulosa cells. The actions of this progesterone-stimulating factor (PSF) were characterized further by purifying it by sequential chromatographies on heparin-agarose, copper chelating-Sepharose and Mono S columns. Several of its effects on granulosa cells were then compared with those of FSH. Like FSH, PSF induced accumulation of progesterone and 20 alpha-dihydroprogesterone in granulosa cell culture media; however, the time course of progesterone accumulation in response to PSF was much slower than that in response to FSH. In contrast to FSH, PSF induced little accumulation of oestrogen. Induction of other differentiated responses was compared by pretreating cultured granulosa cells with either FSH or PSF, and then challenging with LH, prolactin, epidermal growth factor or the beta-adrenergic agonist, isoproterenol. Pretreatment with FSH induced subsequent responsiveness to all four agents, whereas pretreatment with PSF induced subsequent responsiveness only to isoproterenol. These results indicate that granulosa cell responses to PSF are much more limited than are those to FSH and that these responses are characterized by an increase in progestin production.

Concanavalin A - pharmacology

Cells, Cultured

Rats

Spleen - cytology

Prolactin - pharmacology

Rats, Sprague-Dawley

Stimulation, Chemical

Granulosa Cells - drug effects

Granulosa Cells - metabolism

Spleen - chemistry

Follicle Stimulating Hormone - pharmacology

Animals

Isoproterenol - pharmacology

Female

Luteinizing Hormone - pharmacology

Progesterone - biosynthesis

Epidermal Growth Factor - pharmacology

Details

Title: Subtitle: Stimulation of rat granulosa cell progesterone production but not other differentiated functions by a splenocyte-derived factor
Creators: J M Ness - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City 52242, USA
B G Kasson
Resource Type: Journal article
Publication Details: Journal of reproduction & fertility, Vol.105(1), pp.125-133
Publisher: England
DOI: 10.1530/jrf.0.1050125
PMID: 7490703
ISSN: 0022-4251
eISSN: 1471-7899
Grant note: DK-25295 / NIDDK NIH HHS
Language: English
Date published: 09/1995
Academic Unit: Neuroscience and Pharmacology
Record Identifier: 9984040365302771

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