Journal article
Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy
Annals of neurology, Vol.5(2), pp.107-110
02/1979
DOI: 10.1002/ana.410050202
PMID: 426472
Abstract
The influence of the membrane‐stablizing agents diphenylhydantoin (DPH) and orgotein (a drug with superoxide dismutase activity) on protein synthesis was studied in cultured human muscle cells obtained from 7 patients with Duchenne muscular dystrophy (DMD) and 14 controls. The cultures were obtained by dissociation and subsequent plating of cells from biopsied quadriceps muscle. These cultures were then labeled with tritiated leucine in the presence and absence of the membrane‐stabilizing drugs. Total protein synthesis (cpm/mg noncollagen protein) in first‐passage muscle cell cultures from DMD patients showed a 35% decrease, but myosin synthesis revealed normal values. DPH and orgotein increased total protein synthesis by 35 and 50%, respectively, in dystrophic cultured cells, but only by 7 and 8%, respectively, in control cultured cells. Copyright © 1979 American Neurological Association
Details
- Title: Subtitle
- Stimulatory effects of drugs for protein synthesis on muscle cell cultures in duchenne dystrophy
- Creators
- Victor Ionasescu - University of IowaLawrence Z. Stern - University of ArizonaRebeca Ionasescu - University of ArizonaPeter Rubenstein - University of Arizona
- Resource Type
- Journal article
- Publication Details
- Annals of neurology, Vol.5(2), pp.107-110
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/ana.410050202
- PMID
- 426472
- ISSN
- 0364-5134
- eISSN
- 1531-8249
- Number of pages
- 4
- Language
- English
- Date published
- 02/1979
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984288717102771
Metrics
4 Record Views